F3/-2.5CAT | Addgene 25425 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

F3/-2.5CAT

catalog :

25425

citations: 1

Reference
Goldhamer D, Faerman A, Shani M, Emerson C. Regulatory elements that control the lineage-specific expression of myoD. Science. 1992;256:538-42 pubmed

product information

Catalog Number :

25425

Product Name :

F3/-2.5CAT

article :

doi	10.1126/science.1315077
id	3443
pubmed_id	1315077

bacterial resistance :

Ampicillin

cloning :

backbone

pCATdeltaEH

backbone_mutation

backbone_origin

Mellon Lab

backbone_size

4100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

Reporter construct

growth notes :

Fragment 3 was cloned into the XbaI site of -2.5CAT by digesting chMD-13 with NotI and EcoRI, and blunt-end-ligating the fragment into the unique XbaI site. The -2.5 promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 6.8kb. All tk sequences were removed from the CAT vector in the construction of -2.5kb. Between the enhancer and promoter is a portion of pBluescript polylinker that includes BamHI, SpeI and SmaI sites, none of which are unique.

origin :

pi :

alt_names

MyoD

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	XbaI
promoter
sequencing_primer_3	CAT-R
sequencing_primer_5	N/A
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1
gene	MYOD1
id	4654

genbank_ids

mutation

name

Myo D Promoter + F3

shRNA_sequence

size

species

9606

Homo sapiens