This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
-2.5CAT
catalog :
25424
citations: 1
Reference
Goldhamer D, Faerman A, Shani M, Emerson C. Regulatory elements that control the lineage-specific expression of myoD. Science. 1992;256:538-42 pubmed
product information
Catalog Number :
25424
Product Name :
-2.5CAT
article :
doi10.1126/science.1315077
id3443
pubmed_id1315077
bacterial resistance :
Ampicillin
cloning :
backbonepCATdeltaEH
backbone_mutation
backbone_originMellon Lab
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Reporter construct
growth notes :
The -2.5 promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 6.8kb. All tk sequences were removed from the CAT vector in the construction of -2.5kb.
origin :
37
pi :
alt_names
MyoD
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5XbaI
promoter
sequencing_primer_3CAT-R
sequencing_primer_5N/A
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1
geneMYOD1
id4654
genbank_ids
mutation
nameMyo D Promoter
shRNA_sequence
size2700
species
9606
Homo sapiens
tags
locationC terminal on insert
tagCAT
resistance markers :
747
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA