-24GFP | Addgene 25423 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

-24GFP

catalog :

25423

citations: 1

Reference
Yamamoto M, Watt C, Schmidt R, Kuscuoglu U, Miesfeld R, Goldhamer D. Cloning and characterization of a novel MyoD enhancer-binding factor. Mech Dev. 2007;124:715-28 pubmed

product information

Catalog Number :

25423

Product Name :

-24GFP

article :

doi	10.1016/j.mod.2007.07.002
id	3442
pubmed_id	17693064

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-1

backbone_mutation

backbone_origin

backbone_size

4200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

Reporter construct

growth notes :

The MyoDGFP (-24GFP) transgene was constructed by excising 24 kb of human MyoD 5' flanking sequence from -24lacZ (Chen et al., 2001; Addgene plasmid #25422) by digestion with SalI and AscI (AscI linkers were added to the unique XhoI site at the 3' end of human genomic sequences) and inserting into SalI and AscI (produced by linker addition to the unique SmaI site) sites in the polylinker of pEGFP-1 (Clontech). Prior to subcloning, the NotI site just 3' of EGFP was destroyed by blunt-ending with Klenow, and a new NotI site was introduced by linker addition to the AflII site, which lies 3' of SV40 poly sequences.

origin :

pi :

alt_names

MyoD

cloning

clone_method	Restriction Enzyme
cloning_site_3	AscI
cloning_site_5	SalI
promoter
sequencing_primer_3	EGFP-N
sequencing_primer_5	N/A
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1
gene	MYOD1
id	4654

genbank_ids

mutation

name

Myo D 5' flanking sequence

shRNA_sequence

size

24000

species

9606

Homo sapiens