pEGFPm-DAD M1041A | Addgene 25411 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFPm-DAD M1041A

catalog :

25411

citations: 2

Reference
Chiu Y, Medina C, Doyle C, Zhou M, Narahari A, Sandilos J, et al. Deacetylation as a receptor-regulated direct activation switch for pannexin channels. Nat Commun. 2021;12:4482 pubmed publisher
Alberts A. Identification of a carboxyl-terminal diaphanous-related formin homology protein autoregulatory domain. J Biol Chem. 2001;276:2824-30 pubmed

product information

Catalog Number :

25411

Product Name :

pEGFPm-DAD M1041A

article :

doi	10.1074/jbc.M006205200
id	3437
pubmed_id	11035012

bacterial resistance :

Ampicillin

cloning :

backbone

EF-BOS

backbone_mutation

backbone_origin

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The Q1091P mutation in the DAD region was not intentional and may have been a cloning error.

origin :

pi :

alt_names

Diap3 Diaphanous-autoregulatory domain

mDia2 DAD

Diap3 DAD

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3	CCA GGG CAT TAG CCA CAC CAG CCA CCA CTT TCT G
sequencing_primer_5	GCC ATC TAT TGC TTA CAT TTG CTT CTG ACA CAA CTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	4930417P13Rik, Dia2, Diap3, Drf3, p134MDia2
gene	Diaph3
id	56419

genbank_ids

mutation

Alanine substitution at critical residue in DAD that is required for binding to the Dia-inhibitory domain (DID); the M1041A renders it biologically inactive. Q1091P mutation

name

Diap3

shRNA_sequence

size

433

species

10090

Mus musculus