This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPm-DAD
catalog :
25410
citations: 2
Reference
Chiu Y, Medina C, Doyle C, Zhou M, Narahari A, Sandilos J, et al. Deacetylation as a receptor-regulated direct activation switch for pannexin channels. Nat Commun. 2021;12:4482 pubmed publisher
Alberts A. Identification of a carboxyl-terminal diaphanous-related formin homology protein autoregulatory domain. J Biol Chem. 2001;276:2824-30 pubmed
product information
Catalog Number :
25410
Product Name :
pEGFPm-DAD
article :
doi10.1074/jbc.M006205200
id3437
pubmed_id11035012
bacterial resistance :
Ampicillin
cloning :
backboneEF-BOS
backbone_mutation
backbone_origin
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The Q1091P mutation in the DAD region was not intentional and may have been a cloning error.
origin :
37
pi :
alt_names
Diap3 Diaphanous-autoregulatory domain
mDia2 DAD
Diap3 DAD
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3CCA GGG CAT TAG CCA CAC CAG CCA CCA CTT TCT G
sequencing_primer_5GCC ATC TAT TGC TTA CAT TTG CTT CTG ACA CAA CTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases4930417P13Rik, Dia2, Diap3, Drf3, p134MDia2
geneDiaph3
id56419
genbank_ids
mutationmDia2 Amino Acids 1031-1171; Q1091P mutation
nameDiap3
shRNA_sequence
size433
species
10090
Mus musculus
tags
locationN terminal on backbone
tagHis
locationN terminal on backbone
tagMyc
locationN terminal on backbone
tagEGFP
resistance markers :
742
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA