pCMV-Flag-hMcl-1 | Addgene 25392 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV-Flag-hMcl-1

catalog :

25392

citations: 7

Reference
Aka Y, Karakas B, Acikbas U, Basaga H, Gul O, Kutuk O. Kinome-wide RNAi screening for mediators of ABT-199 resistance in breast cancer cells identifies Wee1 as a novel therapeutic target. Int J Biochem Cell Biol. 2021;137:106028 pubmed publisher
Xiao B, Deng X, Lim G, Zhou W, Saw W, Zhou Z, et al. p62-Mediated mitochondrial clustering attenuates apoptosis induced by mitochondrial depolarization. Biochim Biophys Acta Mol Cell Res. 2017;1864:1308-1317 pubmed publisher
Mattoo A, Pandita R, Chakraborty S, Charaka V, Mujoo K, Hunt C, et al. MCL-1 Depletion Impairs DNA Double-Strand Break Repair and Reinitiation of Stalled DNA Replication Forks. Mol Cell Biol. 2017;37: pubmed publisher
Wu D, Wu T, Chen C, Lee H. PAK1 Is a Novel Therapeutic Target in Tyrosine Kinase Inhibitor-Resistant Lung Adenocarcinoma Activated by the PI3K/AKT Signaling Regardless of EGFR Mutation. Clin Cancer Res. 2016;22:5370-5382 pubmed
Lee J, Tang S, Ortiz V, Vo T, Fruman D. MCL-1-independent mechanisms of synergy between dual PI3K/mTOR and BCL-2 inhibition in diffuse large B cell lymphoma. Oncotarget. 2015;6:35202-17 pubmed publisher
Choi Y, Harhaj E. HTLV-1 tax stabilizes MCL-1 via TRAF6-dependent K63-linked polyubiquitination to promote cell survival and transformation. PLoS Pathog. 2014;10:e1004458 pubmed publisher
Ling X, Cao S, Cheng Q, Keefe J, Rustum Y, Li F. A novel small molecule FL118 that selectively inhibits survivin, Mcl-1, XIAP and cIAP2 in a p53-independent manner, shows superior antitumor activity. PLoS ONE. 2012;7:e45571 pubmed publisher

product information

Catalog Number :

25392

Product Name :

pCMV-Flag-hMcl-1

article :

doi
id	3430
pubmed_id

bacterial resistance :

Kanamycin

cloning :

backbone

pCMV-Tag2A

backbone_mutation

backbone_origin

Stratagene

backbone_size

4300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

unpublished There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation. The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRV
cloning_site_5	Bam HI
promoter
sequencing_primer_3
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT
gene	MCL1
id	4170

genbank_ids

NM_021960

mutation

name

MCL1

shRNA_sequence

size

1100

species

9606

Homo sapiens