This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDNA3.1-hMcl-1
catalog :
25375
citations: 20
Reference
Sneyers F, Kerkhofs M, Speelman Rooms F, Welkenhuyzen K, La Rovere R, Shemy A, et al. Intracellular BAPTA directly inhibits PFKFB3, thereby impeding mTORC1-driven Mcl-1 translation and killing MCL-1-addicted cancer cells. Cell Death Dis. 2023;14:600 pubmed publisher
Kim J, Lee D, Pan C, Park S, Oh S, Lee S. Role of phloretin as a sensitizer to TRAIL-induced apoptosis in colon cancer. Oncol Lett. 2022;24:321 pubmed publisher
Shang E, Nguyen T, Shu C, Westhoff M, Karpel Massler G, Siegelin M. Epigenetic Targeting of Mcl-1 Is Synthetically Lethal with Bcl-xL/Bcl-2 Inhibition in Model Systems of Glioblastoma. Cancers (Basel). 2020;12: pubmed publisher
Ye H, Liu Y, Wu K, Luo H, Cui L. AMPK activation overcomes anti-EGFR antibody resistance induced by KRAS mutation in colorectal cancer. Cell Commun Signal. 2020;18:115 pubmed publisher
Xu J, Huang F, Yao Z, Jia C, Xiong Z, Liang H, et al. Inhibition of cyclin E1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression. Cell Commun Signal. 2019;17:85 pubmed publisher
Donadoni M, Cicalese S, Sarkar D, Chang S, Sariyer I. Alcohol exposure alters pre-mRNA splicing of antiapoptotic Mcl-1L isoform and induces apoptosis in neural progenitors and immature neurons. Cell Death Dis. 2019;10:447 pubmed publisher
Burmi R, Maginn E, Gabra H, Stronach E, Wasan H. Combined inhibition of the PI3K/mTOR/MEK pathway induces Bim/Mcl-1-regulated apoptosis in pancreatic cancer cells. Cancer Biol Ther. 2019;20:21-30 pubmed publisher
Luanpitpong S, Poohadsuan J, Samart P, Kiratipaiboon C, Rojanasakul Y, Issaragrisil S. Reactive oxygen species mediate cancer stem-like cells and determine bortezomib sensitivity via Mcl-1 and Zeb-1 in mantle cell lymphoma. Biochim Biophys Acta Mol Basis Dis. 2018;1864:3739-3753 pubmed publisher
Sariyer R, De Simone F, Donadoni M, Hoek J, Chang S, Sariyer I. Alcohol-Mediated Missplicing of Mcl-1 Pre-mRNA is Involved in Neurotoxicity. Alcohol Clin Exp Res. 2017;41:1715-1724 pubmed publisher
Sander B, Xu W, Eilers M, Popov N, Lorenz S. A conformational switch regulates the ubiquitin ligase HUWE1. elife. 2017;6: pubmed publisher
Iyer D, Vartak S, Mishra A, Goldsmith G, Kumar S, Srivastava M, et al. Identification of a novel BCL2-specific inhibitor that binds predominantly to the BH1 domain. FEBS J. 2016;283:3408-37 pubmed publisher
Leclerc G, DeSalvo J, Du J, Gao N, Leclerc G, Lehrman M, et al. Mcl-1 downregulation leads to the heightened sensitivity exhibited by BCR-ABL positive ALL to induction of energy and ER-stress. Leuk Res. 2015;: pubmed publisher
Cerella C, Muller F, Gaigneaux A, Radogna F, Viry E, Chateauvieux S, et al. Early downregulation of Mcl-1 regulates apoptosis triggered by cardiac glycoside UNBS1450. Cell Death Dis. 2015;6:e1782 pubmed publisher
Su J, Tseng P, Hsu C, Tai W, Huang J, Ko C, et al. RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells. Oncotarget. 2014;5:4909-19 pubmed
Pareja F, Macleod D, Shu C, Crary J, Canoll P, Ross A, et al. PI3K and Bcl-2 inhibition primes glioblastoma cells to apoptosis through downregulation of Mcl-1 and Phospho-BAD. Mol Cancer Res. 2014;12:987-1001 pubmed publisher
Tsai A, Pai H, Wang C, Liou J, Teng C, Wang J, et al. In vitro and in vivo anti-tumour effects of MPT0B014, a novel derivative aroylquinoline, and in combination with erlotinib in human non-small-cell lung cancer cells. Br J Pharmacol. 2014;171:122-33 pubmed publisher
Chunhacha P, Pongrakhananon V, Rojanasakul Y, Chanvorachote P. Caveolin-1 regulates Mcl-1 stability and anoikis in lung carcinoma cells. Am J Physiol Cell Physiol. 2012;302:C1284-92 pubmed publisher
Chen C, Liao C, Chang Y, Guh J, Pan S, Teng C. Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines. Cancer Lett. 2012;315:1-11 pubmed publisher
Bhalla S, Evens A, Dai B, Prachand S, Gordon L, Gartenhaus R. The novel anti-MEK small molecule AZD6244 induces BIM-dependent and AKT-independent apoptosis in diffuse large B-cell lymphoma. Blood. 2011;118:1052-61 pubmed publisher
Morel C, Carlson S, White F, Davis R. Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Mol Cell Biol. 2009;29:3845-52 pubmed publisher
product information
Catalog Number :
25375
Product Name :
pCDNA3.1-hMcl-1
article :
doi10.1128/MCB.00279-09
id3428
pubmed_id19433446
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1D V5-His-TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size5514
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation. The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI. The tags on this plasmid are not in frame with hMcl-1.
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3n/a
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT
geneMCL1
id4170
genbank_ids
NM_021960
mutation
nameMCL1
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagV5 and His tag
plasmid copy :
Ulrich Maurer- Glycogen Synthase Kinase-3 Regulates Mitochondrial Outer Membrane Permeabilization and Apoptosis by Destabilization of MCL-1
resistance markers :
106
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA