pCDNA3.1-hMcl-1(S159A) | Addgene 25374 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDNA3.1-hMcl-1(S159A)

catalog :

25374

citations: 2

Reference
Iacovelli S, Ricciardi M, Allegretti M, Mirabilii S, Licchetta R, Bergamo P, et al. Co-targeting of Bcl-2 and mTOR pathway triggers synergistic apoptosis in BH3 mimetics resistant acute lymphoblastic leukemia. Oncotarget. 2015;6:32089-103 pubmed publisher
Morel C, Carlson S, White F, Davis R. Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Mol Cell Biol. 2009;29:3845-52 pubmed publisher

product information

Catalog Number :

25374

Product Name :

pCDNA3.1-hMcl-1(S159A)

article :

doi	10.1128/MCB.00279-09
id	3428
pubmed_id	19433446

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1D V5-His-TOPO

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5514

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D172N D173E mutations. The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI. The tags on this plasmid are not in frame with hMcl-1.

origin :

pi :

alt_names

MCL1

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	n/a
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT
gene	MCL1
id	4170

genbank_ids

NM_021960

mutation

changed serine 159 to alanine

name

hMCL1(S159A)

shRNA_sequence

size

1100

species

9606

Homo sapiens