This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe-Flag hMcl-1(T163A)
catalog :
25373
citations: 1
Reference
Morel C, Carlson S, White F, Davis R. Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Mol Cell Biol. 2009;29:3845-52 pubmed publisher
product information
Catalog Number :
25373
Product Name :
pBabe-Flag hMcl-1(T163A)
article :
doi10.1128/MCB.00279-09
id3428
pubmed_id19433446
bacterial resistance :
Ampicillin
cloning :
backbonepBabe IRES puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation. The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.
origin :
37
pi :
alt_names
MCL1
cloning
clone_methodRestriction Enzyme
cloning_site_3Sal I
cloning_site_5blunt
promoter
sequencing_primer_3pBabe 3'
sequencing_primer_5pBabe 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesBCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT
geneMCL1
id4170
genbank_ids
NM_021960
mutationchanged threonine 163 to alanine
namehMCL1(T163A)
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
106
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA