This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe-Flag hMcl-1
catalog :
25371
citations: 5
Reference
Roders N, Herr F, Ambroise G, Thaunat O, Portier A, Vazquez A, et al. SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production. Front Immunol. 2018;9:787 pubmed publisher
Yoo B, Khan I, Koomson A, Gowda P, Sasazuki T, Shirasawa S, et al. Oncogenic RAS-induced downregulation of ATG12 is required for survival of malignant intestinal epithelial cells. Autophagy. 2018;14:134-151 pubmed publisher
Nikhil K, Shah K. The Cdk5-Mcl-1 axis promotes mitochondrial dysfunction and neurodegeneration in a model of Alzheimer's disease. J Cell Sci. 2017;130:3023-3039 pubmed publisher
Farrugia M, Sharma S, Lin C, McLaughlin S, Vanderbilt D, Ammer A, et al. Regulation of anti-apoptotic signaling by Kruppel-like factors 4 and 5 mediates lapatinib resistance in breast cancer. Cell Death Dis. 2015;6:e1699 pubmed publisher
Morel C, Carlson S, White F, Davis R. Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Mol Cell Biol. 2009;29:3845-52 pubmed publisher
product information
Catalog Number :
25371
Product Name :
pBabe-Flag hMcl-1
article :
doi10.1128/MCB.00279-09
id3428
pubmed_id19433446
bacterial resistance :
Ampicillin
cloning :
backbonepBabe IRES puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
There are a few mismatches between the sequence provided and the existing NCBI sequence. The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI. Addgene NGS did not identify IRES sequence in plasmid.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Sal I
cloning_site_5blunt
promoter
sequencing_primer_3pBabe 3'
sequencing_primer_5pBabe 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesBCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT
geneMCL1
id4170
genbank_ids
NM_021960
mutation
nameMCL1
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
106
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA