This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIR-CDH1 mutUTR
catalog :
25039
citations: 2
Reference
Seashols Williams S, Budd W, Clark G, Wu Q, Daniel R, Dragoescu E, et al. miR-9 Acts as an OncomiR in Prostate Cancer through Multiple Pathways That Drive Tumour Progression and Metastasis. PLoS ONE. 2016;11:e0159601 pubmed publisher
Ma L, Young J, Prabhala H, Pan E, Mestdagh P, Muth D, et al. miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Nat Cell Biol. 2010;12:247-56 pubmed publisher
product information
Catalog Number :
25039
Product Name :
pMIR-CDH1 mutUTR
article :
doi10.1038/ncb2024
id3415
pubmed_id20173740
bacterial resistance :
Ampicillin
cloning :
backbonepMIR-report
backbone_mutation
backbone_originAmbion
backbone_size6470
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
A partial CDH1 3'UTR (819bp) was PCR-amplified from normal human genomic DNA and cloned into the pMIR-report vector. The mutant construct of the CDH1 3 UTR was generated with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).
origin :
37
pi :
alt_names
CDH1
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5SpeI
promoter
sequencing_primer_3M13/pUC Forward
sequencing_primer_5Luc-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesArc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO
geneCDH1
id999
genbank_ids
mutationsubstitution of 6nt within the miR-9 binding site from CAAAGA to GTTTCT.
nameCDH1 mutant 3'UTR
shRNA_sequence
size819
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA