This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIR-9
catalog :
25037
citations: 4
Reference
Mary L, Leclerc D, Labalme A, Bellaud P, Mazaud Guittot S, Dr xe9 ano S, et al. Functional Assessment of a New PBX1 Variant in a 46,XY Fetus with Severe Syndromic Difference of Sexual Development through CRISPR-Cas9 Gene Editing. Genes (Basel). 2023;14: pubmed publisher
Citron F, Segatto I, Musco L, Pellarin I, Rampioni Vinciguerra G, Franchin G, et al. miR-9 modulates and predicts the response to radiotherapy and EGFR inhibition in HNSCC. EMBO Mol Med. 2021;13:e12872 pubmed publisher
Kropivšek K, Pickford J, Carter D. Postnatal dynamics of Zeb2 expression in rat brain: analysis of novel 3' UTR sequence reveals a miR-9 interacting site. J Mol Neurosci. 2014;52:138-47 pubmed publisher
Ma L, Young J, Prabhala H, Pan E, Mestdagh P, Muth D, et al. miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Nat Cell Biol. 2010;12:247-56 pubmed publisher
product information
Catalog Number :
25037
Product Name :
pMIR-9
article :
doi10.1038/ncb2024
id3415
pubmed_id20173740
bacterial resistance :
Ampicillin
cloning :
backbonepMIR-report
backbone_mutation
backbone_originAmbion
backbone_size6470
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
Human miR-9 binding site (the reverse complementary sequence of miR-9) was generated by annealing of 2 oligos (5 -CTAGTTCATACAGCTAGATAACCAAAGAGCTCAGCA-3 (forward) and 5 -AGCTTGCTGAGCTCTTTGGTTATCTAGCTGTATGAA-3 (reverse)), and ligating into pMIR-report.
origin :
37
pi :
alt_names
microRNA 9
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5SpeI
promoter
sequencing_primer_3M13/pUC Forward
sequencing_primer_5Luc-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN9-1, hsa-mir-9-1, miRNA9-1, mir-9-1
geneMIR9-1
id407046
genbank_ids
mutation
namemiR-9 binding site
shRNA_sequence
size36
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA