This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MDH1-PGK-GFP-miR9
catalog :
25036
citations: 5
Reference
Citron F, Segatto I, Musco L, Pellarin I, Rampioni Vinciguerra G, Franchin G, et al. miR-9 modulates and predicts the response to radiotherapy and EGFR inhibition in HNSCC. EMBO Mol Med. 2021;13:e12872 pubmed publisher
Topol A, Zhu S, Hartley B, English J, Hauberg M, Tran N, et al. Dysregulation of miRNA-9 in a Subset of Schizophrenia Patient-Derived Neural Progenitor Cells. Cell Rep. 2016;15:1024-1036 pubmed publisher
Kim D, Hwang I, Muller F, Paik J. Functional regulation of FoxO1 in neural stem cell differentiation. Cell Death Differ. 2015;22:2034-45 pubmed publisher
Gomez G, Volinia S, Croce C, Zanca C, Li M, Emnett R, et al. Suppression of microRNA-9 by mutant EGFR signaling upregulates FOXP1 to enhance glioblastoma tumorigenicity. Cancer Res. 2014;74:1429-39 pubmed publisher
Ma L, Young J, Prabhala H, Pan E, Mestdagh P, Muth D, et al. miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Nat Cell Biol. 2010;12:247-56 pubmed publisher
product information
Catalog Number :
25036
Product Name :
MDH1-PGK-GFP-miR9
article :
doi10.1038/ncb2024
id3415
pubmed_id20173740
bacterial resistance :
Ampicillin
cloning :
backboneMDH1-PGK-GFP 2.0
backbone_mutation
backbone_originChang-Zheng Chen, Addgene plasmid 11375
backbone_size6963
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
The mir-9-3 gene was PCR-amplified from normal human genomic DNA and cloned into the MDH-PGK-GFP 2.0 retroviral vector. It includes the miR-9 stem-loop and ~100bp flanking sequences on both sides.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3H1, tcgctatgtgttctgggaaa
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN9-3, hsa-mir-9-3, miRNA9-3, mir-9-3
geneMIR9-3
id407051
genbank_ids
mutation
namemir-9-3
shRNA_sequence
size313
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA