U6-shRNA pgkCre | Addgene 24971 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

U6-shRNA pgkCre

catalog :

24971

citations: 3

Reference
Gruner H, Zhang Y, Shariati K, Yiv N, Hu Z, Wang Y, et al. SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response. Peerj. 2023;11:e15077 pubmed publisher
Alam H, Li N, Dhar S, Wu S, Lv J, Chen K, et al. HP1γ Promotes Lung Adenocarcinoma by Downregulating the Transcription-Repressive Regulators NCOR2 and ZBTB7A. Cancer Res. 2018;78:3834-3848 pubmed publisher
Young N, Jacks T. Tissue-specific p19Arf regulation dictates the response to oncogenic K-ras. Proc Natl Acad Sci U S A. 2010;107:10184-9 pubmed publisher

product information

Catalog Number :

24971

Product Name :

U6-shRNA pgkCre

article :

doi	10.1073/pnas.1004796107
id	3606
pubmed_id	20479239

bacterial resistance :

Ampicillin

cloning :

backbone

unknown

backbone_mutation

backbone_origin

backbone_size

7705

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

This vector allows for constitutive Cre and shRNA expression. It does not contain any selection cassette. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/jacks-lab/protocols/pSico.html

growth temp :

Stbl3

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	HpaI
promoter
sequencing_primer_3	MSCV-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

shRNA

shRNA_sequence

size

species