This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2 xIGF1
catalog :
24967
citations: 1
Reference
Pera E, Wessely O, Li S, De Robertis E. Neural and head induction by insulin-like growth factor signals. Dev Cell. 2001;1:655-65 pubmed
product information
Catalog Number :
24967
Product Name :
pCS2 xIGF1
article :
doi10.1016/S1534-5807(01)00069-7
id3330
pubmed_id11709186
bacterial resistance :
Ampicillin
cloning :
backbonepCS2
backbone_mutation
backbone_origin
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
mRNA injection sense: cut with NotI, transcribe with Sp6 Anti-sense in situ probe: cut with EcoRI, transcribe with T7 PCR fragment of IGF1 (EcoRI-XhoI) from stage 27 cDNA inserted into pCS2 (EcoRI-XhoI). Sense: 5'AAA GAA TTC ATG GAA ACA AAC AAC AAT Antisense: 5'-TTT CTC GAG TTA CAT TCG GAA TCC TCG
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3T7
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIGF-I, xigf1, igf1-A
geneigf1
id378699
genbank_ids
mutation
nameinsulin-like growth factor 1
shRNA_sequence
size360
species
8355
Xenopus laevis
tags
resistance markers :
657
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA