This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-LC3 (human)
catalog :
24920
citations: 85
Reference
Zhang S, Zeng L, Su B, Yang G, Wang J, Ming S, et al. The glycoprotein 5 of porcine reproductive and respiratory syndrome virus stimulates mitochondrial ROS to facilitate viral replication. MBio. 2023;14:e0265123 pubmed publisher
Ghosh S, Sharma R, Bammidi S, Koontz V, Nemani M, Yazdankhah M, et al. The AKT2/SIRT5/TFEB pathway as a potential therapeutic target in atrophic AMD. bioRxiv. 2023;: pubmed publisher
Naskar M, Parekh V, Abraham M, Alibasic Z, Kim M, Suk G, et al. α-Hemolysin promotes uropathogenic E. coli persistence in bladder epithelial cells via abrogating bacteria-harboring lysosome acidification. PLoS Pathog. 2023;19:e1011388 pubmed publisher
Zhu P, Li J, Jiang X, Zhang S, Zhang Z, Wang Y, et al. A traditional prescription comprising Astragali Radix and Schisandra chinensis Fructus induces apoptosis and protective autophagy in hepatocellular carcinoma cells. J Ethnopharmacol. 2023;312:116548 pubmed publisher
Cohen Kedar S, Shaham Barda E, Rabinowitz K, Keizer D, Abu Taha H, Schwartz S, et al. Human intestinal epithelial cells can internalize luminal fungi via LC3-associated phagocytosis. Front Immunol. 2023;14:1142492 pubmed publisher
Li M, Tripathi Giesgen I, Schulman B, Baumeister W, Wilfling F. In situ snapshots along a mammalian selective autophagy pathway. Proc Natl Acad Sci U S A. 2023;120:e2221712120 pubmed publisher
Zhou X, Cho J, Yi J, Choi K, Park K, Zhu H, et al. Quantification of autophagy flux in isolated mouse skeletal muscle fibers with overexpression of fluorescent protein mCherry-EGFP-LC3. STAR Protoc. 2023;4:101871 pubmed publisher
Hatori Y, Kanda Y, Nonaka S, Nakanishi H, Kitazawa T. ATP13A2 modifies mitochondrial localization of overexpressed TOM20 to autolysosomal pathway. PLoS ONE. 2022;17:e0276823 pubmed publisher
Hao W, Dian M, Zhou Y, Zhong Q, Pang W, Li Z, et al. Autophagy induction promoted by m6A reader YTHDF3 through translation upregulation of FOXO3 mRNA. Nat Commun. 2022;13:5845 pubmed publisher
Paul S, Dansithong W, Gandelman M, Figueroa K, Zu T, Ranum L, et al. Staufen Impairs Autophagy in Neurodegeneration. Ann Neurol. 2022;: pubmed publisher
Kawano D, Pinter K, Chlebowski M, Petralia R, Wang Y, Nechiporuk A, et al. NudC regulated Lis1 stability is essential for the maintenance of dynamic microtubule ends in axon terminals. iScience. 2022;25:105072 pubmed publisher
Kawalec P, Martens M, Field J, Mughal W, Caymo A, Chapman D, et al. Differential impact of doxorubicin dose on cell death and autophagy pathways during acute cardiotoxicity. Toxicol Appl Pharmacol. 2022;453:116210 pubmed publisher
Wang J, Onogi Y, Krueger M, Oeckl J, Karlina R, Singh I, et al. PAT2 regulates vATPase assembly and lysosomal acidification in brown adipocytes. Mol Metab. 2022;61:101508 pubmed publisher
Belaidi A, Masaldan S, Southon A, Kalinowski P, Acevedo K, Appukuttan A, et al. Apolipoprotein E potently inhibits ferroptosis by blocking ferritinophagy. Mol Psychiatry. 2022;: pubmed publisher
Yu P, Fu P, Zeng L, Qi Y, Li X, Wang Q, et al. EGCG Restricts PRRSV Proliferation by Disturbing Lipid Metabolism. Microbiol Spectr. 2022;10:e0227621 pubmed publisher
Zhou J, He H, Zhang J, Liu X, Yao W, Li C, et al. ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation. Autophagy. 2022;:1-23 pubmed publisher
Campisi D, Desrues L, Dembélé K, Mutel A, Parment R, Gandolfo P, et al. The core autophagy protein ATG9A controls dynamics of cell protrusions and directed migration. J Cell Biol. 2022;221: pubmed publisher
Abokyi S, Shan S, Lam C, Catral K, Pan F, Chan H, et al. Targeting Lysosomes to Reverse Hydroquinone-Induced Autophagy Defects and Oxidative Damage in Human Retinal Pigment Epithelial Cells. Int J Mol Sci. 2021;22: pubmed publisher
Cho K, Shin S, Moon H, Kim B, Ro S. Knockdown of Atg7 suppresses Tumorigenesis in a murine model of liver cancer. Transl Oncol. 2021;14:101158 pubmed publisher
Ma K, Fokkens M, Reggiori F, Mari M, Verbeek D. Parkinson's disease-associated VPS35 mutant reduces mitochondrial membrane potential and impairs PINK1/Parkin-mediated mitophagy. Transl Neurodegener. 2021;10:19 pubmed publisher
Santerre M, Arjona S, Allen C, Callen S, Buch S, Sawaya B. HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. Autophagy. 2021;17:1768-1782 pubmed publisher
Xu S, Zhou Z, Peng X, Tao X, Zhou P, Zhang K, et al. EBV-LMP1 promotes radioresistance by inducing protective autophagy through BNIP3 in nasopharyngeal carcinoma. Cell Death Dis. 2021;12:344 pubmed publisher
Wu H, Voeltz G. Reticulon-3 Promotes Endosome Maturation at ER Membrane Contact Sites. Dev Cell. 2021;56:52-66.e7 pubmed publisher
Xia T, Zhang J, Zhou C, Li Y, Duan W, Zhang B, et al. 20(S)-Ginsenoside Rh2 displays efficacy against T-cell acute lymphoblastic leukemia through the PI3K/Akt/mTOR signal pathway. J Ginseng Res. 2020;44:725-737 pubmed publisher
Abokyi S, Shan S, To C, Chan H, Tse D. Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells. Oxid Med Cell Longev. 2020;2020:5296341 pubmed publisher
Wu J, Yu X, Xue K, Wu J, Wang R, Xie X, et al. The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease. Oxid Med Cell Longev. 2020;2020:8735249 pubmed publisher
Li L, Liu J, Gao G, Zhang K, Song Y, Li H. Puerarin 6″-O-xyloside suppressed HCC via regulating proliferation, stemness, and apoptosis with inhibited PI3K/AKT/mTOR. Cancer Med. 2020;9:6399-6410 pubmed publisher
Yu H, Zhou X, Wang Y, Huang X, Yang J, Zeng J, et al. Nicotinamide N-methyltransferase inhibits autophagy induced by oxidative stress through suppressing the AMPK pathway in breast cancer cells. Cancer Cell Int. 2020;20:191 pubmed publisher
Liu X, Zhong L, Li P, Zhao P. MicroRNA-100 Enhances Autophagy and Suppresses Migration and Invasion of Renal Cell Carcinoma Cells via Disruption of NOX4-Dependent mTOR Pathway. Clin Transl Sci. 2020;: pubmed publisher
Goebel T, Mausbach S, Tuermer A, Eltahir H, Winter D, Gieselmann V, et al. Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7. Mol Cell Proteomics. 2020;19:1120-1131 pubmed publisher
Miao Q, Xu Y, Yin H, Zhang H, Ye J. KRT8 phosphorylation regulates the epithelial-mesenchymal transition in retinal pigment epithelial cells through autophagy modulation. J Cell Mol Med. 2020;24:3217-3228 pubmed publisher
Najafi S, Abo Ali E, Dukhande V. Methods for Studying TNFα-Induced Autophagy. Methods Mol Biol. 2020;2108:131-146 pubmed publisher
Luo X, Donnelly C, Gong W, Heath B, Hao Y, Donnelly L, et al. HPV16 drives cancer immune escape via NLRX1-mediated degradation of STING. J Clin Invest. 2020;130:1635-1652 pubmed publisher
Garva R, Thepmalee C, Yasamut U, Sudsaward S, Guazzelli A, Rajendran R, et al. Sirtuin Family Members Selectively Regulate Autophagy in Osteosarcoma and Mesothelioma Cells in Response to Cellular Stress. Front Oncol. 2019;9:949 pubmed publisher
Guo H, Li Y, Liu G, Jiang Y, Shen S, Bi R, et al. A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells. Nat Commun. 2019;10:4066 pubmed publisher
Zhang W, Zhuang N, Liu X, He L, He Y, Mahinthichaichan P, et al. The metabolic regulator Lamtor5 suppresses inflammatory signaling via regulating mTOR-mediated TLR4 degradation. Cell Mol Immunol. 2019;: pubmed publisher
Emami A, Shojaei S, da Silva Rosa S, Aghaei M, Samiei E, Vosoughi A, et al. Mechanisms of simvastatin myotoxicity: The role of autophagy flux inhibition. Eur J Pharmacol. 2019;862:172616 pubmed publisher
Safiulina D, Kuum M, Choubey V, Gogichaishvili N, Liiv J, Hickey M, et al. Miro proteins prime mitochondria for Parkin translocation and mitophagy. EMBO J. 2019;38: pubmed publisher
Miao Q, Xu Y, Zhang H, Xu P, Ye J. Cigarette smoke induces ROS mediated autophagy impairment in human corneal epithelial cells. Environ Pollut. 2019;245:389-397 pubmed publisher
Moghadam A, da Silva Rosa S, Samiei E, Alizadeh J, Field J, Kawalec P, et al. Autophagy modulates temozolomide-induced cell death in alveolar Rhabdomyosarcoma cells. Cell Death Discov. 2018;4:52 pubmed publisher
Yang F, Wang F, Liu Y, Wang S, Li X, Huang Y, et al. Sulforaphane induces autophagy by inhibition of HDAC6-mediated PTEN activation in triple negative breast cancer cells. Life Sci. 2018;213:149-157 pubmed publisher
Zhang T, Guo L, Wang Y, Yang Y. Macroautophagy Regulates Nuclear NOTCH1 Activity Through Multiple p62 Binding Sites. IUBMB Life. 2018;70:985-994 pubmed publisher
Torralba D, Baixauli F, Villarroya Beltri C, Fernandez Delgado I, Latorre Pellicer A, Acin Perez R, et al. Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts. Nat Commun. 2018;9:2658 pubmed publisher
Wang Y, Mandal A, Son Y, Pratheeshkumar P, Wise J, Wang L, et al. Roles of ROS, Nrf2, and autophagy in cadmium-carcinogenesis and its prevention by sulforaphane. Toxicol Appl Pharmacol. 2018;353:23-30 pubmed publisher
Tan Y, Sansanaphongpricha K, Xie Y, Donnelly C, Luo X, Heath B, et al. Mitigating SOX2-potentiated Immune Escape of Head and Neck Squamous Cell Carcinoma with a STING-inducing Nanosatellite Vaccine. Clin Cancer Res. 2018;24:4242-4255 pubmed publisher
Kuo W, Ho T, Chiang S, Pai P, Lin J, Lin D, et al. Rab9-dependent autophagy is required for the IGF-IIR triggering mitophagy to eliminate damaged mitochondria. J Cell Physiol. 2018;233:7080-7091 pubmed publisher
Yang R, Yang E, Shen L, Modlin R, Shen H, Chen Z. IL-12+IL-18 Cosignaling in Human Macrophages and Lung Epithelial Cells Activates Cathelicidin and Autophagy, Inhibiting Intracellular Mycobacterial Growth. J Immunol. 2018;200:2405-2417 pubmed publisher
Liu Y, Li D, He Z, Liu Q, Wu J, Guan X, et al. Inhibition of autophagy-attenuated calcium oxalate crystal-induced renal tubular epithelial cell injury in vivo and in vitro. Oncotarget. 2018;9:4571-4582 pubmed publisher
Liao L, Zhuang X, Li W, Su Q, Zhao J, Liu Y. Polysaccharide from Fuzi protects against Ox‑LDL‑induced calcification of human vascular smooth muscle cells by increasing autophagic activity. Mol Med Rep. 2018;17:5109-5115 pubmed publisher
Al Qatati A, Aliwaini S. Combined pitavastatin and dacarbazine treatment activates apoptosis and autophagy resulting in synergistic cytotoxicity in melanoma cells. Oncol Lett. 2017;14:7993-7999 pubmed publisher
Mishra S, Gao Y, Deng Y, Chalfant C, Hinchcliffe E, Brown R. CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation. Autophagy. 2018;14:862-879 pubmed publisher
Kondratskyi A, Kondratska K, Vanden Abeele F, Gordienko D, Dubois C, Toillon R, et al. Ferroquine, the next generation antimalarial drug, has antitumor activity. Sci Rep. 2017;7:15896 pubmed publisher
Alizadeh J, Shojaei S, Sepanjnia A, Hashemi M, Eftekharpour E, Ghavami S. Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells. Methods Mol Biol. 2019;1854:87-103 pubmed publisher
Tang Z, Cao W, Guo X, Dai X, Lu J, Chen X, et al. Identification of a novel autophagic inhibitor cepharanthine to enhance the anti-cancer property of dacomitinib in non-small cell lung cancer. Cancer Lett. 2018;412:1-9 pubmed publisher
Linares J, Cordes T, Duran A, Reina Campos M, Valencia T, Ahn C, et al. ATF4-Induced Metabolic Reprograming Is a Synthetic Vulnerability of the p62-Deficient Tumor Stroma. Cell Metab. 2017;26:817-829.e6 pubmed publisher
Medvedev R, Ploen D, Spengler C, Elgner F, Ren H, Bunten S, et al. HCV-induced oxidative stress by inhibition of Nrf2 triggers autophagy and favors release of viral particles. Free Radic Biol Med. 2017;110:300-315 pubmed publisher
Wang L, Gundelach J, Bram R. Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme. Cell Death Dis. 2017;8:e2807 pubmed publisher
Tang Z, Cao W, Su M, Chen X, Lu J. Osimertinib induces autophagy and apoptosis via reactive oxygen species generation in non-small cell lung cancer cells. Toxicol Appl Pharmacol. 2017;321:18-26 pubmed publisher
Liu Y, Gong W, Yang Z, Zhou X, Gong C, Zhang T, et al. Quercetin induces protective autophagy and apoptosis through ER stress via the p-STAT3/Bcl-2 axis in ovarian cancer. Apoptosis. 2017;22:544-557 pubmed publisher
Li W, Reeb A, Lin B, Subramanian P, Fey E, Knoverek C, et al. Heat Shock-induced Phosphorylation of TAR DNA-binding Protein 43 (TDP-43) by MAPK/ERK Kinase Regulates TDP-43 Function. J Biol Chem. 2017;292:5089-5100 pubmed publisher
Liu Y, Weng Y, Lin H, Tang S, Chen C, Liang C, et al. Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells. Sci Rep. 2017;7:41437 pubmed publisher
Shyu R, Wang C, Wu C, Chen M, Lee M, Wang L, et al. Tazarotene-Induced Gene 1 Enhanced Cervical Cell Autophagy through Transmembrane Protein 192. Mol Cells. 2016;39:877-887 pubmed publisher
Coly P, Perzo N, Le Joncour V, Lecointre C, Schouft M, Desrues L, et al. Chemotactic G protein-coupled receptors control cell migration by repressing autophagosome biogenesis. Autophagy. 2016;12:2344-2362 pubmed
Wei R, Lin S, Wu W, Chen L, Li C, Chen H, et al. A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells. Toxicol Appl Pharmacol. 2016;311:88-98 pubmed publisher
Mossine V, Waters J, Chance D, Mawhinney T. Transient Proteotoxicity of Bacterial Virulence Factor Pyocyanin in Renal Tubular Epithelial Cells Induces ER-Related Vacuolation and Can Be Efficiently Modulated by Iron Chelators. Toxicol Sci. 2016;154:403-415 pubmed
Cagalinec M, Liiv M, Hodurova Z, Hickey M, Vaarmann A, Mandel M, et al. Role of Mitochondrial Dynamics in Neuronal Development: Mechanism for Wolfram Syndrome. PLoS Biol. 2016;14:e1002511 pubmed publisher
Ryu D, Mouchiroud L, Andreux P, Katsyuba E, Moullan N, Nicolet Dit Félix A, et al. Urolithin A induces mitophagy and prolongs lifespan in C. elegans and increases muscle function in rodents. Nat Med. 2016;22:879-88 pubmed publisher
Mahil S, Twelves S, Farkas K, Setta Kaffetzi N, Burden A, Gach J, et al. AP1S3 Mutations Cause Skin Autoinflammation by Disrupting Keratinocyte Autophagy and Up-Regulating IL-36 Production. J Invest Dermatol. 2016;136:2251-2259 pubmed publisher
O Donnell J, Jackson J, Robinson M. Transient Oxygen/Glucose Deprivation Causes a Delayed Loss of Mitochondria and Increases Spontaneous Calcium Signaling in Astrocytic Processes. J Neurosci. 2016;36:7109-27 pubmed publisher
Tang Z, Chen X, Wang Z, Chai K, Wang Y, Xu X, et al. Induction of C/EBP homologous protein-mediated apoptosis and autophagy by licochalcone A in non-small cell lung cancer cells. Sci Rep. 2016;6:26241 pubmed publisher
Xia T, Wang J, Wang Y, Wang Y, Cai J, Wang M, et al. Inhibition of autophagy potentiates anticancer property of 20(S)-ginsenoside Rh2 by promoting mitochondria-dependent apoptosis in human acute lymphoblastic leukaemia cells. Oncotarget. 2016;7:27336-49 pubmed publisher
Lopes V, Loitto V, Audinot J, Bayat N, Gutleb A, Cristobal S. Dose-dependent autophagic effect of titanium dioxide nanoparticles in human HaCaT cells at non-cytotoxic levels. J Nanobiotechnology. 2016;14:22 pubmed publisher
Lei Y, Kansy B, Li J, Cong L, Liu Y, Trivedi S, et al. EGFR-targeted mAb therapy modulates autophagy in head and neck squamous cell carcinoma through NLRX1-TUFM protein complex. Oncogene. 2016;35:4698-707 pubmed publisher
Liu X, He Y, Yang Q, Huang W, Liu Z, Ye G, et al. Induction of autophagy and apoptosis by miR-148a through the sonic hedgehog signaling pathway in hepatic stellate cells. Am J Cancer Res. 2015;5:2569-89 pubmed
Tang Z, Zhang L, Li T, Lu J, Ma D, Leung C, et al. Glycyrrhetinic acid induces cytoprotective autophagy via the inositol-requiring enzyme 1α-c-Jun N-terminal kinase cascade in non-small cell lung cancer cells. Oncotarget. 2015;6:43911-26 pubmed publisher
Pandhare J, Dash S, Jones B, Villalta F, Dash C. A Novel Role of Proline Oxidase in HIV-1 Envelope Glycoprotein-induced Neuronal Autophagy. J Biol Chem. 2015;290:25439-51 pubmed publisher
Metge B, Mitra A, Chen D, Shevde L, Samant R. N-Myc and STAT Interactor regulates autophagy and chemosensitivity in breast cancer cells. Sci Rep. 2015;5:11995 pubmed publisher
Rui Y, Xu Z, Chen Z, Zhang S. The GST-BHMT assay reveals a distinct mechanism underlying proteasome inhibition-induced macroautophagy in mammalian cells. Autophagy. 2015;11:812-32 pubmed publisher
Buratta S, Urbanelli L, Ferrara G, Sagini K, Goracci L, Emiliani C. A role for the autophagy regulator Transcription Factor EB in amiodarone-induced phospholipidosis. Biochem Pharmacol. 2015;95:201-9 pubmed publisher
Yeh E, Abt M, Hill E. Regulation of cell survival by HUNK mediates breast cancer resistance to HER2 inhibitors. Breast Cancer Res Treat. 2015;149:91-8 pubmed publisher
Wang M, Shim J, Li R, Dang Y, He Q, Das M, et al. Identification of an old antibiotic clofoctol as a novel activator of unfolded protein response pathways and an inhibitor of prostate cancer. Br J Pharmacol. 2014;171:4478-89 pubmed publisher
Zhang Q, Si S, Schoen S, Chen J, Jin X, Wu G. Suppression of autophagy enhances preferential toxicity of paclitaxel to folliculin-deficient renal cancer cells. J Exp Clin Cancer Res. 2013;32:99 pubmed publisher
Liu H, Yu S, Zhang H, Xu J. Angiogenesis impairment in diabetes: role of methylglyoxal-induced receptor for advanced glycation endproducts, autophagy and vascular endothelial growth factor receptor 2. PLoS ONE. 2012;7:e46720 pubmed publisher
Duan L, Motchoulski N, Danzer B, Davidovich I, Shariat Madar Z, Levenson V. Prolylcarboxypeptidase regulates proliferation, autophagy, and resistance to 4-hydroxytamoxifen-induced cytotoxicity in estrogen receptor-positive breast cancer cells. J Biol Chem. 2011;286:2864-76 pubmed publisher
Lee I, Cao L, Mostoslavsky R, Lombard D, Liu J, Bruns N, et al. A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy. Proc Natl Acad Sci U S A. 2008;105:3374-9 pubmed publisher
product information
Catalog Number :
24920
Product Name :
pEGFP-LC3 (human)
article :
doi10.1073/pnas.0712145105
id3374
pubmed_id18296641
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C2
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression of LC3 fused to EGFP
origin :
37
pi :
alt_names
microtubule-associated protein 1 light chain 3
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-F, EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
BC067797
mutation
nameLC3
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
146
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA