human p53-(311-393) | Addgene 24869 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

human p53-(311-393)

catalog :

24869

citations: 1

Reference
Ayed A, Mulder F, Yi G, Lu Y, Kay L, Arrowsmith C. Latent and active p53 are identical in conformation. Nat Struct Biol. 2001;8:756-60 pubmed

product information

Catalog Number :

24869

Product Name :

human p53-(311-393)

article :

doi	10.1038/nsb0901-756
id	3363
pubmed_id	11524676

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX-2TK

backbone_mutation

backbone_origin

backbone_size

5000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

E.Coli strain for epression: BL21DE3 Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight. NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NdeI
promoter
sequencing_primer_3
sequencing_primer_5	pGEX5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

Contains aa 311-393.

name

p53

shRNA_sequence

size

246

species

9606

Homo sapiens