GFAP-Cre | Addgene 24704 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

GFAP-Cre

catalog :

24704

citations: 3

Reference
Hara T, Verma I. Modeling Gliomas Using Two Recombinases. Cancer Res. 2019;79:3983-3991 pubmed publisher
Sofroniew M. Transgenic techniques for cell ablation or molecular deletion to investigate functions of astrocytes and other GFAP-expressing cell types. Methods Mol Biol. 2012;814:531-44 pubmed publisher
Bush T, Puvanachandra N, Horner C, Polito A, Ostenfeld T, Svendsen C, et al. Leukocyte infiltration, neuronal degeneration, and neurite outgrowth after ablation of scar-forming, reactive astrocytes in adult transgenic mice. Neuron. 1999;23:297-308 pubmed

product information

Catalog Number :

24704

Product Name :

GFAP-Cre

article :

doi	10.1016/S0896-6273(00)80781-3
id	3333
pubmed_id	10399936

bacterial resistance :

Ampicillin

cloning :

backbone

GFAP-HSV-TK

backbone_mutation

backbone_origin

Michael Sofroniew (Addgene Plasmid# 24703)

backbone_size

18000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A modified Cre (including an artificial intron, nuclear localization signal and HSV-TK polyA was derived from pACN (Bunting et al., (1999) Genes Dev 13: 1524-1528) to create GFAP-Cre as described in Garcia et al., (2004) Nat Neurosci. 7:1233-1241. Adaptors were used to clone Cre into the NotI/SalI sites of the GFAP-HSV-TK plasmid described in Bush et al. (1998) Cell 93:189-201. Details of the original GFAP (glial fibrillary acidic protein) promoter cassette (GFAP-lacZ) can be found in Johnson et al. (1995) Glia 13:174-184.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	NotI
promoter	GFAP (glial fibrillary acidic protein)
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

Cre

shRNA_sequence

size

species

100

Synthetic