This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
OKSIM
catalog :
24603
citations: 10
Reference
Kaur P, Otgonbaatar A, Ramamoorthy A, Chua E, Harmston N, Gruber J, et al. Combining stem cell rejuvenation and senescence targeting to synergistically extend lifespan. Aging (Albany NY). 2022;14:8270-8291 pubmed publisher
Cha Y, Kim T, Jeon J, Jang Y, Kim P, Lopes C, et al. SIRT2 regulates mitochondrial dynamics and reprogramming via MEK1-ERK-DRP1 and AKT1-DRP1 axes. Cell Rep. 2021;37:110155 pubmed publisher
Moshref M, Questa M, Lopez Cervantes V, Sears T, Greathouse R, Crawford C, et al. Panobinostat Effectively Increases Histone Acetylation and Alters Chromatin Accessibility Landscape in Canine Embryonic Fibroblasts but Does Not Enhance Cellular Reprogramming. Front Vet Sci. 2021;8:716570 pubmed publisher
Lubanska D, Qemo I, Byrne M, Matthews K, Fifield B, Brown J, et al. The cyclin-like protein SPY1 overrides reprogramming induced senescence through EZH2 mediated H3K27me3. Stem Cells. 2021;39:1688-1700 pubmed publisher
Manousiouthakis E, Chen Y, Cairns D, Pollard R, Gerlovin K, Dente M, et al. Bioengineered In Vitro Enteric Nervous System. J Tissue Eng Regen Med. 2019;: pubmed publisher
Cairns D, Giordano J, Conte S, Levin M, Kaplan D. Ivermectin Promotes Peripheral Nerve Regeneration during Wound Healing. ACS Omega. 2018;3:12392-12402 pubmed publisher
Cairns D, Boorgu D, Levin M, Kaplan D. Niclosamide rescues microcephaly in a humanized in vivo model of Zika infection using human induced neural stem cells. Biol Open. 2018;7: pubmed publisher
Wang L, Chen Y, Guan C, Zhao Z, Li Q, Yang J, et al. Using low-risk factors to generate non-integrated human induced pluripotent stem cells from urine-derived cells. Stem Cell Res Ther. 2017;8:245 pubmed publisher
Kim S, Oceguera Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, et al. KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015;4:727-43 pubmed publisher
Ross P, Suhr S, Rodriguez R, Chang E, Wang K, Siripattarapravat K, et al. Human-induced pluripotent stem cells produced under xeno-free conditions. Stem Cells Dev. 2010;19:1221-9 pubmed publisher
product information
Catalog Number :
24603
Product Name :
OKSIM
article :
doi10.1089/scd.2009.0459
id3297
pubmed_id20030562
bacterial resistance :
Ampicillin
cloning :
backbonepSin4-EF2-IRES-Pur
backbone_mutation
backbone_origin
backbone_size6777
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Cre/Lox
growth notes :
Constructed by Steven T. Suhr, MSU Cellular Reprogramming Laboratory
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Pme I
cloning_site_5Mlu I
promoter
sequencing_primer_3
sequencing_primer_5EF-1a Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
aliasesEZF, GKLF
geneKLF4
id9314
genbank_ids
mutationOct4-2A-KLF4-2A-Sox2-IRES-Myc
nameOct4 KLF4 Sox2 c-Myc
shRNA_sequence
size5600
species
9606
Homo sapiens
tags
plasmid copy :
The pSin4-EF2-IRES-Pur was obtained from Addgene (James Thomson's laboratory) in the form of pSin-EF2-Oct4-Pur. The WPRE/LoxP LTR portion was obtained from Addgene in the form of pWPT-GFP (Luigi Naldini's laboratory). Transgene ORFs were cloned from human factor cDNAs.
resistance markers :
731
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA