pFloxin-IRES-Ofd1-Myc-A80T | Addgene 24575 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFloxin-IRES-Ofd1-Myc-A80T

catalog :

24575

citations: 1

Reference
Singla V, Romaguera Ros M, Garcia Verdugo J, Reiter J. Ofd1, a human disease gene, regulates the length and distal structure of centrioles. Dev Cell. 2010;18:410-24 pubmed publisher

product information

Catalog Number :

24575

Product Name :

pFloxin-IRES-Ofd1-Myc-A80T

article :

doi	10.1016/j.devcel.2009.12.022
id	3284
pubmed_id	20230748

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript

backbone_mutation

backbone_origin

backbone_size

4873

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mouse Targeting

Cre/Lox

growth notes :

Mutation reported in: Four novel mutations in the OFD1 (Cxorf5) gene in Finnish patients with oral-facial-digital syndrome 1. Rakkolainen A, Ala-Mello S, Kristo P, Orpana A, J rvel I. J Med Genet. 2002 Apr;39(4):292-6. No abstract available. PMID: 11950863

growth temp :

Stbl2 (Invitrogen) or other RecA- strain 37 degrees in LB media

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	tcggtgcacatgctttacat
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ORF2, Cxorf5, DXGgc7e
gene	Ofd1
id	237222

genbank_ids

NM_177429

mutation

Alanine 80 changed to threonine (codon GCA to ACA)

name

Ofd1

shRNA_sequence

size

3087

species

10090

Mus musculus