pFloxin-IRES-Ofd1-Myc-S75F | Addgene 24574 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFloxin-IRES-Ofd1-Myc-S75F

catalog :

24574

citations: 1

Reference
Singla V, Romaguera Ros M, Garcia Verdugo J, Reiter J. Ofd1, a human disease gene, regulates the length and distal structure of centrioles. Dev Cell. 2010;18:410-24 pubmed publisher

product information

Catalog Number :

24574

Product Name :

pFloxin-IRES-Ofd1-Myc-S75F

article :

doi	10.1016/j.devcel.2009.12.022
id	3284
pubmed_id	20230748

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript

backbone_mutation

backbone_origin

backbone_size

4873

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mouse Targeting

Cre/Lox

growth notes :

Mutation reported in: OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells. Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA. J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504

growth temp :

Stbl2 (Invitrogen) or other RecA- strain 37 degrees in LB media

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	tcggtgcacatgctttacat
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ORF2, Cxorf5, DXGgc7e
gene	Ofd1
id	237222

genbank_ids

NM_177429

mutation

serine 75 changed to phenylalanine (codon TCT changed to TTT)

name

Ofd1

shRNA_sequence

size

3087

species

10090

Mus musculus