This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFloxin-IRES-Ofd1-Myc
catalog :
24559
citations: 1
Reference
Singla V, Hunkapiller J, Santos N, Seol A, Norman A, Wakenight P, et al. Floxin, a resource for genetically engineering mouse ESCs. Nat Methods. 2010;7:50-2 pubmed publisher
product information
Catalog Number :
24559
Product Name :
pFloxin-IRES-Ofd1-Myc
article :
doi10.1038/nmeth.1406
id3282
pubmed_id19966808
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript
backbone_mutation
backbone_origin
backbone_size4501
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mouse Targeting
Cre/Lox
growth notes :
Also see the following article: Ofd1, a human disease gene, regulates the length and distal structure of centrioles. Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF. Dev Cell. 2010 Mar 16;18(3):410-24.PMID: 20230748
growth temp :
Stbl2 (Invitrogen) or other RecA- strain 37 degrees in LB media
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5NheI
promoter
sequencing_primer_3
sequencing_primer_5tcggtgcacatgctttacat
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesORF2, Cxorf5, DXGgc7e
geneOfd1
id237222
genbank_ids
NM_177429
mutation
nameOfd1
shRNA_sequence
size3550
species
10090
Mus musculus
tags
locationC terminal on insert
tagMyc
resistance markers :
715
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA