This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV/SB10 DDE
catalog :
24552
citations: 1
Reference
Ivics Z, Hackett P, Plasterk R, Izsvak Z. Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell. 1997;91:501-10 pubmed
product information
Catalog Number :
24552
Product Name :
pCMV/SB10 DDE
article :
doi10.1016/S0092-8674(00)80436-5
id3281
pubmed_id9390559
bacterial resistance :
Ampicillin
cloning :
backbonepCMVbeta
backbone_mutationpCMV/SB10 (Addgene plasmid # 24551) was cut with MscI to remove 322 bp of the transposase coding region which deleted much of the catalytic DDE domain and disrupted the reading frame by introducing a premature translational termination codon.
backbone_originClontech
backbone_size3712
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
SBdDDE
SB10dDDE
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NotI
promoterCMV
sequencing_primer_3M13 Reverse
sequencing_primer_5M13 Forward
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
mutationremoves 322 bp of the transposase-coding region
nameSleeping Beauty delta DDE
shRNA_sequence
size698
species
tags
resistance markers :
811
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA