This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe-puro-IMS-RP
catalog :
24535
citations: 4
Reference
Chandrasekharan A, Varadarajan S, Lekshmi A, Lupitha S, Darvin P, Chandrasekhar L, et al. A high-throughput real-time in vitro assay using mitochondrial targeted roGFP for screening of drugs targeting mitochondria. Redox Biol. 2019;20:379-389 pubmed publisher
Nerlich A, Mieth M, Letsiou E, Fatykhova D, Zscheppang K, Imai Matsushima A, et al. Pneumolysin induced mitochondrial dysfunction leads to release of mitochondrial DNA. Sci Rep. 2018;8:182 pubmed publisher
Coutu J, Ryerson M, Bugert J, Brian Nichols D. The Molluscum Contagiosum Virus protein MC163 localizes to the mitochondria and dampens mitochondrial mediated apoptotic responses. Virology. 2017;505:91-101 pubmed publisher
Albeck J, Burke J, Spencer S, Lauffenburger D, Sorger P. Modeling a snap-action, variable-delay switch controlling extrinsic cell death. PLoS Biol. 2008;6:2831-52 pubmed publisher
product information
Catalog Number :
24535
Product Name :
pBabe-puro-IMS-RP
article :
doi10.1371/journal.pbio.0060299
id3274
pubmed_id19053173
bacterial resistance :
Ampicillin
cloning :
backbonepBabe-puro
backbone_mutation
backbone_originBob Weinberg, Addgene plasmid 1764
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
IMS-RP, a reporter for MOMP that localizes to the mitochondrial intermembrane space, was constructed by PCR of bp 1-147 of Smac-1, ligation to the 5 end of monomeric RFP-1, and cloning into pBabe-Puro. See https://www.ncbi.nlm.nih.gov/pubmed/18406323 for more information
origin :
37
pi :
alt_names
DIABLO, SMAC
mitochondrial intermembrane space reporter protein (IMS-RP)
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDFNA64, SMAC
geneDIABLO
id56616
genbank_ids
mutationDeleted residues 56-239
nameDiablo homolog
shRNA_sequence
size867
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmRFP1
resistance markers :
738
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA