This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAdEasy-mRFP-gsk3 beta S9A
catalog :
24521
citations: 2
Reference
Lakshmanan J, Zhang B, Nweze I, Du Y, Harbrecht B. Glycogen synthase kinase 3 regulates IL-1β mediated iNOS expression in hepatocytes by down-regulating c-Jun. J Cell Biochem. 2015;116:133-41 pubmed publisher
Kumar P, Lyle K, Gierke S, Matov A, Danuser G, Wittmann T. GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009;184:895-908 pubmed publisher
product information
Catalog Number :
24521
Product Name :
pAdEasy-mRFP-gsk3 beta S9A
article :
doi10.1083/jcb.200901042
id3230
pubmed_id19289791
bacterial resistance :
Kanamycin
cloning :
backbonepAdEasy-1
backbone_mutation
backbone_originStratagene, ATCC
backbone_size33414
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Adenoviral
growth notes :
mRFP-GSK3beta S9A was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1. AdEasy is a registered trademark of the Johns Hopkins University.
origin :
37
pi :
alt_names
GSK3beta
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5CMV-F, EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneGSK3B
id2932
genbank_ids
mutationS9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
nameGSK3beta(S9A)
shRNA_sequence
size2000
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmRFP
plasmid copy :
cDNA obtained from Niels Galjart
resistance markers :
709
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA