pFLAG UBR1 SBX | Addgene 24506 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFLAG UBR1 SBX

catalog :

24506

citations: 3

Reference
Fenteany G, Sharma G, Gaur P, Borics A, Wéber E, Kiss E, et al. A series of xanthenes inhibiting Rad6 function and Rad6-Rad18 interaction in the PCNA ubiquitination cascade. iScience. 2022;25:104053 pubmed publisher
Pan M, Zheng Q, Wang T, Liang L, Mao J, Zuo C, et al. Structural insights into Ubr1-mediated N-degron polyubiquitination. Nature. 2021;600:334-338 pubmed publisher
Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A. Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway. J Biol Chem. 2008;283:24011-28 pubmed publisher

product information

Catalog Number :

24506

Product Name :

pFLAG UBR1 SBX

article :

doi	10.1074/jbc.M802583200
id	2427
pubmed_id	18566452

bacterial resistance :

Ampicillin

cloning :

backbone

Yeplac 181

backbone_mutation

backbone_origin

backbone_size

5741

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

SBX stands for SalI, BamHI, XhoI. SalI is 15 bp before ATG codon of UBR1ORF. BamHI and XhoI are right after the stop codon of the UBR1ORF. The plasmid expresses UBR1 which is N-terminally Flag tagged and has clean C-terminus. Note that it does not have NcoI site upstream of ATG codon. Active in degrading N-end rule substrates.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter
sequencing_primer_3	M13pUC-Rev
sequencing_primer_5	yADH1-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YGR184C, PTR1
gene	UBR1
id	853096

genbank_ids

mutation

name

UBR1

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae