sq5 | Addgene 24499 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

sq5

catalog :

24499

citations: 2

Reference
Zhang B, Sun N, Mu X, Zhi L, Zhai L, Jiang Y, et al. G Protein Alpha S Subunit Promotes Cell Proliferation of Renal Cell Carcinoma with Involvement of Protein Kinase A Signaling. DNA Cell Biol. 2017;36:237-242 pubmed publisher
Conklin B, Herzmark P, Ishida S, Voyno Yasenetskaya T, Sun Y, Farfel Z, et al. Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Mol Pharmacol. 1996;50:885-90 pubmed

product information

Catalog Number :

24499

Product Name :

sq5

article :

doi
id	3261
pubmed_id	8863834

bacterial resistance :

Ampicillin and Tetracycline

cloning :

backbone

pcDNA1

backbone_mutation

backbone_origin

backbone_size

4000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.

growth temp :

Plasmid contains SupF. Grow in TOP10/P3 cells.

origin :

pi :

alt_names

G protein alpha subunit

GNAS

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BglII
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6
gene	Gnas
id	24896

genbank_ids

mutation

This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV).

name

Gs alpha

shRNA_sequence

size

species

10116

Rattus norvegicus