pcDNA3.1hSIRT3_H248Y_HA | Addgene 24492 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.1hSIRT3_H248Y_HA

catalog :

24492

citations: 2

Reference
Sawant Dessai A, Domínguez M, Chen U, Hasper J, Prechtl C, Yu C, et al. Transcriptional Repression of SIRT3 Potentiates Mitochondrial Aconitase Activation to Drive Aggressive Prostate Cancer to the Bone. Cancer Res. 2021;81:50-63 pubmed publisher
Hirschey M, Shimazu T, Goetzman E, Jing E, Schwer B, Lombard D, et al. SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Nature. 2010;464:121-5 pubmed publisher

product information

Catalog Number :

24492

Product Name :

pcDNA3.1hSIRT3_H248Y_HA

article :

doi	10.1038/nature08778
id	3257
pubmed_id	20203611

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

SIRT3 contains a mutation at amino acid Q32H. This mutation is present in the first 100 amino acids of SIRT3 which serve as its mitochondrial import signal. A peptide corresponding to the first 100 aa of SIRT3 is normally cleaved once the protein has been imported into the mitochondria and the active form of SIRT3 in the mitochondrial matrix therefore lacks this part of the protein. Therefore the mutation should not modify SIRT3 activity.

origin :

pi :

alt_names

SIRT3

SIRT3-H248Y

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	SIR2L3
gene	SIRT3
id	23410

genbank_ids

NM_012239

mutation

H248Y

name

Human Sirtuin 3 catalytically inactive mutant

shRNA_sequence

size

1000

species

9606

Homo sapiens