CMV::SypHy A4 | Addgene 24478 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CMV::SypHy A4

catalog :

24478

citations: 11

Reference
Hao Y, Toulm xe9 E, K xf6 nig B, Rosenmund C, Plested A. Targeted sensors for glutamatergic neurotransmission. elife. 2023;12: pubmed publisher
Dentoni G, Naia L, Ankarcrona M. Mitochondria-Endoplasmic Reticulum Interplay Regulates Exo-Cytosis in Human Neuroblastoma Cells. Cells. 2022;11: pubmed publisher
Dagar S, Teng Z, Gottmann K. Transsynaptic N-Cadherin Adhesion Complexes Control Presynaptic Vesicle and Bulk Endocytosis at Physiological Temperature. Front Cell Neurosci. 2021;15:713693 pubmed publisher
Jiang Z, Li W, Yao L, Saed B, Rao Y, Grewe B, et al. TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis. elife. 2021;10: pubmed publisher
Hughes A, Appel B. Oligodendrocytes express synaptic proteins that modulate myelin sheath formation. Nat Commun. 2019;10:4125 pubmed publisher
Yao C, Liu Y, Lee I, Wang Y, Wu P. A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis. PLoS Biol. 2017;15:e2000931 pubmed publisher
Jackson R, Burrone J. Visualizing Presynaptic Calcium Dynamics and Vesicle Fusion with a Single Genetically Encoded Reporter at Individual Synapses. Front Synaptic Neurosci. 2016;8:21 pubmed publisher
Rost B, Schneider F, Grauel M, Wozny C, Bentz C, Blessing A, et al. Optogenetic acidification of synaptic vesicles and lysosomes. Nat Neurosci. 2015;18:1845-1852 pubmed publisher
Kawaguchi S, Sakaba T. Control of inhibitory synaptic outputs by low excitability of axon terminals revealed by direct recording. Neuron. 2015;85:1273-88 pubmed publisher
Herman M, Ackermann F, Trimbuch T, Rosenmund C. Vesicular glutamate transporter expression level affects synaptic vesicle release probability at hippocampal synapses in culture. J Neurosci. 2014;34:11781-91 pubmed publisher
Granseth B, Odermatt B, Royle S, Lagnado L. Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses. Neuron. 2006;51:773-86 pubmed

product information

Catalog Number :

24478

Product Name :

CMV::SypHy A4

article :

doi	10.1016/j.neuron.2006.08.029
id	3255
pubmed_id	16982422

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

3952

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The depositing lab notes that the Xba1 and Bcl1 unique restriction digestion sites could be methylated and blocked if the plasmid is grown or maintained in dam+ bacteria. Also note that in the Addgene-generated map below, the feature listed as GFP(12-708) is actually pH sensitive pHluorin. There is a mutation S446T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.

origin :

pi :

alt_names

Rat Synaptophysin-pHluorin

cloning

clone_method	Restriction Enzyme
cloning_site_3	Not1
cloning_site_5	Xho1
promoter
sequencing_primer_3
sequencing_primer_5	cctctacaaatgtggtatggc
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	Syp1
gene	Syp
id	24804

genbank_ids

mutation

name

SypHy

shRNA_sequence

size

1665

species

10116

Rattus norvegicus