This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PCLP-2/NT-IgG (104)
catalog :
24423
citations: 1
Reference
Sassetti C, van Zante A, Rosen S. Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins. J Biol Chem. 2000;275:9001-10 pubmed
product information
Catalog Number :
24423
Product Name :
PCLP-2/NT-IgG (104)
article :
doi10.1074/jbc.275.12.9001
id3249
pubmed_id10722749
bacterial resistance :
Ampicillin
cloning :
backboneIg/pEG-BOS
backbone_mutation
backbone_origin
backbone_size7300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
PCR from PCLP-2 clone G11H9-11b with primers (CS36 and CS38). Insert had NheI and BclI sites compatible with XbaI and BamHI in vector. Can NOT be excised. The IgG1 tag was not confirmed in sequencing, but the lab has tested it functionally.
origin :
37
pi :
alt_names
Endoglycan
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI/BclI
cloning_site_5XbaI/NheI
promoter
sequencing_primer_3
sequencing_primer_5EF-1a Forward
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesPODLX2
genePODXL2
id50512
genbank_ids
mutationN-terminal (acidic) domain of PCLP-2 (1-630 bp) fused to Human IgG1 Fc
namePCLP-2
shRNA_sequence
size630
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHuman IgG1 Fc
resistance markers :
424
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA