This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-GAL80
catalog :
24347
citations: 1
Reference
Potter C, Tasic B, Russler E, Liang L, Luo L. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell. 2010;141:536-48 pubmed publisher
product information
Catalog Number :
24347
Product Name :
pCMV-GAL80
article :
doi10.1016/j.cell.2010.02.025
id3234
pubmed_id20434990
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1-Myc-His-A
backbone_mutation
backbone_originInvitrogen
backbone_size5493
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
and a lab vector, pCA-GAL80 as the template. The PCR product was cloned using BamHI and NotI into pCDNA3.1-myc- His-A. A stop codon precedes the Myc and His tags.
(CGGTTAACGCGGCCGCttataaactataatgcgagata
ttgctaacg),
GAL80 cDNA was PCR amplified using primers PR511 (CCCCGGATCCCAACatggactacaacaagagatcttcgg) and PR512
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYML051W
geneGAL80
id854954
genbank_ids
mutation
nameGAL80
shRNA_sequence
size1300
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
304
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA