This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAc-mCh-Tub
catalog :
24288
citations: 2
Reference
Villars A, Matamoro Vidal A, Levillayer F, Levayer R. Microtubule disassembly by caspases is an important rate-limiting step of cell extrusion. Nat Commun. 2022;13:3632 pubmed publisher
Vale R, Spudich J, Griffis E. Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. J Cell Biol. 2009;186:727-38 pubmed publisher
product information
Catalog Number :
24288
Product Name :
pAc-mCh-Tub
article :
doi10.1083/jcb.200902083
id3221
pubmed_id19720876
bacterial resistance :
Ampicillin
cloning :
backbonepAc-mCherryW
backbone_mutation
backbone_origin
backbone_size7952
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
Tub84b cDNA was cloned, inserted into pENTR-D-Topo and the insert was transferred into pAC-mCherry-W using LR cloning.
origin :
37
pi :
alt_names
Alpha-Tub84B
Alpha-Tubulin at 84B
CG1913
cloning
clone_methodRestriction Enzyme
cloning_site_3AttR2
cloning_site_5AttR1
promoter
sequencing_primer_3
sequencing_primer_5RFP Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG1913, 1t, ALPHA 84C, Alpha-Tubulin84B, BEST:LD32507, CG1913, DM1alpha, DTA1, Dmel CG1913, T, TBA1_DROME, Talpha1, Tub, Tub 84B, Tub1C, Tuba84B, Tubalpha1, a-tub84B, a1t, aTub84B, alpha-Tub, alpha-Tub84B, alpha-tub, alpha-tub84B, alpha-tubulin, alpha-tubulin 84B, alpha1, alpha1t, alpha1tub, alpha1tub84B, alpha84B, alphaT, alphaTUB, alphaTUB84B, alphaTub, alphaTub1, alphaTub84, alphat-1, alphatub, alphatub84B, anon-EST:Liang-1.59, anon-EST:Liang-2.30, chr3R:2914276. 2914416, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tub, tubA84B, tubalpha1, tubulin
genealphaTub84B
id40848
genbank_ids
NM_057424
mutation
nameAlpha-Tubulin
shRNA_sequence
size1353
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagmCherry
resistance markers :
543
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA