This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMT-Diaphanous-GFP
catalog :
24287
citations: 1
Reference
Vale R, Spudich J, Griffis E. Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. J Cell Biol. 2009;186:727-38 pubmed publisher
product information
Catalog Number :
24287
Product Name :
pMT-Diaphanous-GFP
article :
doi10.1083/jcb.200902083
id3221
pubmed_id19720876
bacterial resistance :
Ampicillin
cloning :
backbonepMT-WG
backbone_mutation
backbone_origin
backbone_size6786
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
origin :
37
pi :
alt_names
CG1768
Dia
cloning
clone_methodRestriction Enzyme
cloning_site_3AttR2
cloning_site_5AttR1
promoter
sequencing_primer_3
sequencing_primer_5M13 Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG1768, 38E.16, CG1768, DIA, Dia, Diaphanous, Dias, Dmel CG1768, l(2)k07135, ms(2)04138
genedia
id35340
genbank_ids
NM_165341
mutation
nameDiaphanous
shRNA_sequence
size3276
species
7227
Drosophila melanogaster
tags
locationC terminal on backbone
tagGFP
plasmid copy :
I received the cDNA from the lab of Mark Peifer at UNC. I amplified the cDNA and inserted it into the pENTR D Topo vector and then ligated it into the pMT-WG vector using LR cloning.
resistance markers :
543
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA