This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMT-Mad2-GFP
catalog :
24284
citations: 1
Reference
Griffis E, Stuurman N, Vale R. Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J Cell Biol. 2007;177:1005-15 pubmed
product information
Catalog Number :
24284
Product Name :
pMT-Mad2-GFP
article :
doi10.1083/jcb.200702062
id3220
pubmed_id17576797
bacterial resistance :
Ampicillin
cloning :
backbonepMT-WG
backbone_mutation
backbone_origin
backbone_size6786
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
This cDNA was amplified from a library, ligated into pENTR-D-Topo and then the pENTR insert was ligated into pMT-WG.
origin :
37
pi :
alt_names
CG17498
cloning
clone_methodRestriction Enzyme
cloning_site_3AttR2
cloning_site_5AttR1
promoter
sequencing_primer_3
sequencing_primer_5M13 Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG17498, CG17498, Dmel CG17498, MAD2, Mad2
genemad2
id38656
genbank_ids
NM_139734
mutation
nameMad2
shRNA_sequence
size624
species
7227
Drosophila melanogaster
tags
locationC terminal on backbone
tagGFP
resistance markers :
543
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA