This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMT-GFP-p150Dynactin
catalog :
24283
citations: 1
Reference
Griffis E, Stuurman N, Vale R. Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J Cell Biol. 2007;177:1005-15 pubmed
product information
Catalog Number :
24283
Product Name :
pMT-GFP-p150Dynactin
article :
doi10.1083/jcb.200702062
id3220
pubmed_id17576797
bacterial resistance :
Ampicillin
cloning :
backbonepMT-GW
backbone_mutation
backbone_originDrosophila Gateway Vector Collection
backbone_size5943
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
Dr. Goshima made a C-terminal GFP p150 plasmid and Vale lab used his pENTR vector to make an N-terminal fusion using Gateway cloning. Y1238H mutation detected in Addgene's BGH-rev sequence is not likely to affect function.
origin :
37
pi :
alt_names
Glued
CG9206
cloning
clone_methodGateway Cloning
cloning_site_3AttR2
cloning_site_5AttR1
promoter
sequencing_primer_3
sequencing_primer_5pMT-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG9206, CG9206, DCTN1, DYNA_DROME, Dmel CG9206, FCP-D, Gl, Glu, Glued, P150, P150A, P150[Glued], gl, p150, p150/Glued, p150/glued, p150[Glued], p150[glued], t1
geneDCTN1-p150
id39536
genbank_ids
NM_079337
mutation
namep150 Dynactin
shRNA_sequence
size3795
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagGFP
plasmid copy :
Gohta Goshima
resistance markers :
543
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA