This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI NL4-3 Nef-HA-WT
catalog :
24162
citations: 5
Reference
Castro Gonzalez S, Chen Y, Benjamin J, Shi Y, Serra Moreno R. Residues T48 and A49 in HIV-1 NL4-3 Nef are responsible for the counteraction of autophagy initiation, which prevents the ubiquitin-dependent degradation of Gag through autophagosomes. Retrovirology. 2021;18:33 pubmed publisher
Castro Gonzalez S, Shi Y, Colomer Lluch M, Song Y, Mowery K, Almodovar S, et al. HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner. Autophagy. 2020;:1-25 pubmed publisher
Lasso G, Mayer S, Winkelmann E, Chu T, Elliot O, PatiƱo Galindo J, et al. A Structure-Informed Atlas of Human-Virus Interactions. Cell. 2019;178:1526-1541.e16 pubmed publisher
McNamara R, Costantini L, Myers T, Schouest B, Maness N, Griffith J, et al. Nef Secretion into Extracellular Vesicles or Exosomes Is Conserved across Human and Simian Immunodeficiency Viruses. MBio. 2018;9: pubmed publisher
Geleziunas R, Xu W, Takeda K, Ichijo H, Greene W. HIV-1 Nef inhibits ASK1-dependent death signalling providing a potential mechanism for protecting the infected host cell. Nature. 2001;410:834-8 pubmed
product information
Catalog Number :
24162
Product Name :
pCI NL4-3 Nef-HA-WT
article :
doi10.1038/35071111
id3193
pubmed_id11298454
bacterial resistance :
Ampicillin
cloning :
backbonepCl
backbone_mutation
backbone_originPromega
backbone_size4006
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
NL4-3 is a wild-type Nef allele.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoter
sequencing_primer_3EBV_rev
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesHIV1gp9
genenef
id156110
genbank_ids
mutation
nameNef
shRNA_sequence
size700
species
HIV
tags
locationC terminal on backbone
tagHA
resistance markers :
176
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA