This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI-SEP-NRI
catalog :
23999
citations: 7
Reference
Elmasri M, Hunter D, Winchester G, Bates E, Aziz W, Van Der Does D, et al. Common synaptic phenotypes arising from diverse mutations in the human NMDA receptor subunit GluN2A. Commun Biol. 2022;5:174 pubmed publisher
Li S, Raychaudhuri S, Lee S, Brockmann M, Wang J, Kusick G, et al. Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses. Nat Commun. 2021;12:677 pubmed publisher
Woo D, Hur Y, Jang M, Justin Lee C, Park M. Inhibitors of synaptic vesicle exocytosis reduce surface expression of postsynaptic glutamate receptors. Anim Cells Syst (Seoul). 2020;24:341-348 pubmed publisher
Zhu M, Cortese G, Waites C. Parkinson's disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons. BMC Biol. 2018;16:100 pubmed publisher
Hodges J, Vilchez S, Asmussen H, Whitmore L, Horwitz A. ?-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons. PLoS ONE. 2014;9:e101770 pubmed publisher
Wang N, Chen L, Cheng N, Zhang J, Tian T, Lu W. Active calcium/calmodulin-dependent protein kinase II (CaMKII) regulates NMDA receptor mediated postischemic long-term potentiation (i-LTP) by promoting the interaction between CaMKII and NMDA receptors in ischemia. Neural Plast. 2014;2014:827161 pubmed publisher
Kopec C, Li B, Wei W, Boehm J, Malinow R. Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. J Neurosci. 2006;26:2000-9 pubmed
product information
Catalog Number :
23999
Product Name :
pCI-SEP-NRI
article :
doi10.1523/JNEUROSCI.3918-05.2006
id3145
pubmed_id16481433
bacterial resistance :
Ampicillin
cloning :
backbonepCI
backbone_mutation
backbone_origin
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.
origin :
37
pi :
alt_names
NMDA 1 isoform 1a precursor
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGluN1, NMDAR1, NR1
geneGrin1
id24408
genbank_ids
NM_017010.2
NP_058706.1
mutation
nameNR1
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagNR1 signal sequence (aa1-20)
locationN terminal on backbone
tagSEP (superecliptic pHluorin)
resistance markers :
671
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA