This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI-SEP_NR2B
catalog :
23998
citations: 11
Reference
Boehringer S, Johnston T, Kwapisz L, VandeVord P, Costa B. CNS4 causes subtype-specific changes in agonist efficacy and reversal potential of permeant cations in NMDA receptors. Pharmacol Res Perspect. 2023;11:e01107 pubmed publisher
Costa B, Kwapisz L, Mehrkens B, Bledsoe D, Vacca B, Johnston T, et al. A glutamate concentration-biased allosteric modulator potentiates NMDA-induced ion influx in neurons. Pharmacol Res Perspect. 2021;9:e00859 pubmed publisher
Hevesi Z, Zelena D, Romanov R, Hanics J, Ignácz A, Zambon A, et al. Secretagogin marks amygdaloid PKCδ interneurons and modulates NMDA receptor availability. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Akkuratov E, Westin L, Vázquez Juárez E, De Marothy M, Melnikova A, Blom H, et al. Ouabain Modulates the Functional Interaction Between Na,K-ATPase and NMDA Receptor. Mol Neurobiol. 2020;: pubmed publisher
Zhang Z, Bai H, Guo Z, Li H, Diao X, Zhang T, et al. Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn. Sci Signal. 2020;13: pubmed publisher
Zhu M, Cortese G, Waites C. Parkinson's disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons. BMC Biol. 2018;16:100 pubmed publisher
Yang L, Bai H, Zhang Z, Liu J, Suo Z, Yang X, et al. Disruption of SHP1/NMDA receptor signaling in spinal cord dorsal horn alleviated inflammatory pain. Neuropharmacology. 2018;137:104-113 pubmed publisher
Warikoo N, Brunwasser S, Benz A, Shu H, Paul S, Lewis M, et al. Positive Allosteric Modulation as a Potential Therapeutic Strategy in Anti-NMDA Receptor Encephalitis. J Neurosci. 2018;38:3218-3229 pubmed publisher
Stanic J, Carta M, Eberini I, Pelucchi S, Marcello E, Genazzani A, et al. Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nat Commun. 2015;6:10181 pubmed publisher
Park E, Chi S, Park D. Activity-dependent modulation of the interaction between CaMKII? and Abi1 and its involvement in spine maturation. J Neurosci. 2012;32:13177-88 pubmed
Kopec C, Li B, Wei W, Boehm J, Malinow R. Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. J Neurosci. 2006;26:2000-9 pubmed
product information
Catalog Number :
23998
Product Name :
pCI-SEP_NR2B
article :
doi10.1523/JNEUROSCI.3918-05.2006
id3145
pubmed_id16481433
bacterial resistance :
Ampicillin
cloning :
backbonepCI
backbone_mutation
backbone_origin
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See attached map for additional plasmid features. The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5NheI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGluN2B
geneGrin2b
id24410
genbank_ids
EDM01617.1
mutation
nameNR2B
shRNA_sequence
size6300
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagNR2B signal sequence (aa1-31)
locationN terminal on backbone
tagSEP (superecliptic pHluorin)
resistance markers :
671
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA