This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTreTight-Htt94Q-CFP
catalog :
23966
citations: 12
Reference
Powell J, Lim C, Krishnan R, McCallister T, Saporito Magriñá C, Zeballos M, et al. Targeted gene silencing in the nervous system with CRISPR-Cas13. Sci Adv. 2022;8:eabk2485 pubmed publisher
Paquette M, El Houjeiri L, C Zirden L, Puustinen P, Blanchette P, Jeong H, et al. AMPK-dependent phosphorylation is required for transcriptional activation of TFEB and TFE3. Autophagy. 2021;:1-19 pubmed publisher
Seervai R, Jangid R, Karki M, Tripathi D, Jung S, Kearns S, et al. The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase. Sci Adv. 2020;6: pubmed publisher
Ekman F, Ojala D, Adil M, Lopez P, Schaffer D, Gaj T. CRISPR-Cas9-Mediated Genome Editing Increases Lifespan and Improves Motor Deficits in a Huntington's Disease Mouse Model. Mol Ther Nucleic Acids. 2019;17:829-839 pubmed publisher
Ham S, Kim H, Hwang S, Kang H, Yun S, Kim S, et al. Cell-Based Screen Using Amyloid Mimic β23 Expression Identifies Peucedanocoumarin III as a Novel Inhibitor of α-Synuclein and Huntingtin Aggregates. Mol Cells. 2019;42:480-494 pubmed publisher
Ito Ishida A, Yamalanchili H, Shao Y, BAKER S, Heckman L, Lavery L, et al. Genome-wide distribution of linker histone H1.0 is independent of MeCP2. Nat Neurosci. 2018;21:794-798 pubmed publisher
Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
Grimm J, English B, Choi H, Muthusamy A, Mehl B, Dong P, et al. Bright photoactivatable fluorophores for single-molecule imaging. Nat Methods. 2016;13:985-988 pubmed publisher
Chandrasekaran K, Sathyanarayanan A, Karunagaran D. MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells. Br J Cancer. 2016;115:741-51 pubmed publisher
Li L, Liu H, Dong P, Li D, Legant W, Grimm J, et al. Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. elife. 2016;5: pubmed publisher
Zhu X, Zhang L, Kao Y, Xu F, Min W. A tunable fluorescent timer method for imaging spatial-temporal protein dynamics using light-driven photoconvertible protein. J Biophotonics. 2015;8:226-32 pubmed publisher
Maynard C, Böttcher C, Ortega Z, Smith R, Florea B, Diaz Hernandez M, et al. Accumulation of ubiquitin conjugates in a polyglutamine disease model occurs without global ubiquitin/proteasome system impairment. Proc Natl Acad Sci U S A. 2009;106:13986-91 pubmed publisher
product information
Catalog Number :
23966
Product Name :
pTreTight-Htt94Q-CFP
article :
doi10.1073/pnas.0906463106
id3138
pubmed_id19666572
bacterial resistance :
Ampicillin
cloning :
backbonepTRE-tight
backbone_mutation
backbone_originClontech
backbone_size2605
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
There are 96Q repeats and an Argenine in the middle of the repeat.
origin :
37
pi :
alt_names
Htt
HttQ94
Htt polyQ
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHD, IT15, LOMARS
geneHTT
id3064
genbank_ids
mutation94 polyQ repeat
nameN-terminal fragment Huntingtin Q94
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagCFP
resistance markers :
102
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA