This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCherry-Parkin
catalog :
23956
citations: 31
Reference
Waters C, Angenent S, Altschuler S, Wu L. A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit. Cell Rep. 2023;42:113260 pubmed publisher
Read T, Cisterna B, Skruber K, Ahmadieh S, Lindamood H, Vitriol J, et al. The actin binding protein profilin 1 is critical for mitochondria function. bioRxiv. 2023;: pubmed publisher
Quiles J, Najor R, Gonzalez E, Jeung M, Liang W, Burbach S, et al. Deciphering functional roles and interplay between Beclin1 and Beclin2 in autophagosome formation and mitophagy. Sci Signal. 2023;16:eabo4457 pubmed publisher
Kocaturk N, Peker N, Eberhart K, Akkoç Y, Deveci G, Dengjel J, et al. Novel protein complexes containing autophagy and UPS components regulate proteasome-dependent PARK2 recruitment onto mitochondria and PARK2-PARK6 activity during mitophagy. Cell Death Dis. 2022;13:947 pubmed publisher
Chen K, Ernst P, Liu X, Zhou L. Optogenetic Studies of Mitochondria. Methods Mol Biol. 2022;2501:311-324 pubmed publisher
Li J, Dang X, Franco A, Dorn G. Reciprocal Regulation of Mitofusin 2-Mediated Mitophagy and Mitochondrial Fusion by Different PINK1 Phosphorylation Events. Front Cell Dev Biol. 2022;10:868465 pubmed publisher
Franci L, Tubita A, Bertolino F, Palma A, Cannino G, Settembre C, et al. MAPK15 protects from oxidative stress-dependent cellular senescence by inducing the mitophagic process. Aging Cell. 2022;:e13620 pubmed publisher
Fan Y, Cheng Z, Mao L, Xu G, Li N, Zhang M, et al. PINK1/TAX1BP1-directed mitophagy attenuates vascular endothelial injury induced by copper oxide nanoparticles. J Nanobiotechnology. 2022;20:149 pubmed publisher
Li T, Oasa S, Ciruela F, Terenius L, Vukojevic V, Svenningsson P. Cytosolic GPR37, but not GPR37L1, multimerization and its reversal by Parkin: A live cell imaging study. FASEB J. 2021;35:e22055 pubmed publisher
Strobbe D, Pecorari R, Conte O, Minutolo A, Hendriks C, Wiezorek S, et al. NH-sulfoximine: A novel pharmacological inhibitor of the mitochondrial F1 Fo -ATPase, which suppresses viability of cancerous cells. Br J Pharmacol. 2020;: pubmed publisher
Chen Q, Lei J, Bao J, Wang H, Hao W, Li L, et al. BRCA1 Deficiency Impairs Mitophagy and Promotes Inflammasome Activation and Mammary Tumor Metastasis. Adv Sci (Weinh). 2020;7:1903616 pubmed publisher
Liu X, Liao X, Rao X, Wang B, Zhang J, Xu G, et al. The lysosomal membrane protein LAMP-2 is dispensable for PINK1/Parkin-mediated mitophagy. FEBS Lett. 2020;594:823-840 pubmed publisher
Essandoh K, Wang X, Huang W, Deng S, Gardner G, Mu X, et al. Tumor susceptibility gene 101 ameliorates endotoxin-induced cardiac dysfunction by enhancing Parkin-mediated mitophagy. J Biol Chem. 2019;294:18057-18068 pubmed publisher
Ernst P, Xu N, Qu J, Chen H, Goldberg M, Darley Usmar V, et al. Precisely Control Mitochondria with Light to Manipulate Cell Fate Decision. Biophys J. 2019;117:631-645 pubmed publisher
Zhang Z, Wang N, Ma Q, Chen Y, Yao L, Zhang L, et al. Somatic and germline mutations in the tumor suppressor gene PARK2 impair PINK1/Parkin-mediated mitophagy in lung cancer cells. Acta Pharmacol Sin. 2019;: pubmed publisher
Castagnaro S, Chrisam M, Cescon M, Braghetta P, Grumati P, Bonaldo P. Extracellular Collagen VI Has Prosurvival and Autophagy Instructive Properties in Mouse Fibroblasts. Front Physiol. 2018;9:1129 pubmed publisher
Zhang X, Pellegrini P, Saei A, Hillert E, Mazurkiewicz M, Olofsson M, et al. The deubiquitinase inhibitor b-AP15 induces strong proteotoxic stress and mitochondrial damage. Biochem Pharmacol. 2018;156:291-301 pubmed publisher
Kruppa A, Kishi Itakura C, Masters T, Rorbach J, Grice G, Kendrick Jones J, et al. Myosin VI-Dependent Actin Cages Encapsulate Parkin-Positive Damaged Mitochondria. Dev Cell. 2018;44:484-499.e6 pubmed publisher
Pearson G, Chai B, Vozheiko T, Liu X, Kandarpa M, Piper R, et al. Clec16a, Nrdp1, and USP8 Form a Ubiquitin-Dependent Tripartite Complex That Regulates β-Cell Mitophagy. Diabetes. 2018;67:265-277 pubmed publisher
Bonilla Porras A, Arevalo Arbelaez A, Alzate Restrepo J, Velez Pardo C, Jimenez Del Rio M. PARKIN overexpression in human mesenchymal stromal cells from Wharton's jelly suppresses 6-hydroxydopamine-induced apoptosis: Potential therapeutic strategy in Parkinson's disease. Cytotherapy. 2018;20:45-61 pubmed publisher
Hoshino A, Matoba S. Observation of Parkin-Mediated Mitophagy in Pancreatic β-Cells. Methods Mol Biol. 2018;1759:41-46 pubmed publisher
Sauvat A, Zhou H, Leduc M, Gomes da Silva L, Bezu L, Muller K, et al. Automated Analysis of Fluorescence Colocalization: Application to Mitophagy. Methods Enzymol. 2017;588:219-230 pubmed publisher
Philip F, Ha E, Seeliger M, Frohman M. Measuring Phospholipase D Enzymatic Activity Through Biochemical and Imaging Methods. Methods Enzymol. 2017;583:309-325 pubmed publisher
Sargsyan A, Cai J, Fandino L, Labasky M, Forostyan T, Colosimo L, et al. Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single fluorescent biosensor. Sci Rep. 2015;5:12397 pubmed publisher
Zhang Q, Kuang H, Chen C, Yan J, Do Umehara H, Liu X, et al. The kinase Jnk2 promotes stress-induced mitophagy by targeting the small mitochondrial form of the tumor suppressor ARF for degradation. Nat Immunol. 2015;16:458-66 pubmed publisher
Grenier K, Kontogiannea M, Fon E. Short mitochondrial ARF triggers Parkin/PINK1-dependent mitophagy. J Biol Chem. 2014;289:29519-30 pubmed publisher
Hamacher Brady A, Choe S, Krijnse Locker J, Brady N. Intramitochondrial recruitment of endolysosomes mediates Smac degradation and constitutes a novel intrinsic apoptosis antagonizing function of XIAP E3 ligase. Cell Death Differ. 2014;21:1862-76 pubmed publisher
Kim S, Khan M, Quan J, Till A, Subramani S, Siddiqui A. Hepatitis B virus disrupts mitochondrial dynamics: induces fission and mitophagy to attenuate apoptosis. PLoS Pathog. 2013;9:e1003722 pubmed publisher
Hofmeister Brix A, Kollmann K, Langer S, Schultz J, Lenzen S, Baltrusch S. Identification of the ubiquitin-like domain of midnolin as a new glucokinase interaction partner. J Biol Chem. 2013;288:35824-39 pubmed publisher
Gómez Sánchez R, Gegg M, Bravo San Pedro J, Niso Santano M, Alvarez Erviti L, Pizarro Estrella E, et al. Mitochondrial impairment increases FL-PINK1 levels by calcium-dependent gene expression. Neurobiol Dis. 2014;62:426-40 pubmed publisher
Narendra D, Tanaka A, Suen D, Youle R. Parkin is recruited selectively to impaired mitochondria and promotes their autophagy. J Cell Biol. 2008;183:795-803 pubmed publisher
product information
Catalog Number :
23956
Product Name :
mCherry-Parkin
article :
doi10.1083/jcb.200809125
id3134
pubmed_id19029340
bacterial resistance :
Kanamycin
cloning :
backbonemCherry-C1
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
first ATG is deleted
growth strain :
Mammalian expression of human Park2 fused to mCherry
origin :
37
pi :
alt_names
Parkin
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAR-JP, LPRS2, PARK2, PDJ
genePRKN
id5071
genbank_ids
NM_004562
mutation
namePark2
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmCherry
resistance markers :
604
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA