This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YFP-Parkin
catalog :
23955
citations: 33
Reference
Kocaturk N, Peker N, Eberhart K, Akkoç Y, Deveci G, Dengjel J, et al. Novel protein complexes containing autophagy and UPS components regulate proteasome-dependent PARK2 recruitment onto mitochondria and PARK2-PARK6 activity during mitophagy. Cell Death Dis. 2022;13:947 pubmed publisher
Imberechts D, Kinnart I, Wauters F, Terbeek J, Manders L, Wierda K, et al. DJ-1 is an essential downstream mediator in PINK1/parkin-dependent mitophagy. Brain. 2022;145:4368-4384 pubmed publisher
Danese A, Patergnani S, Maresca A, Peron C, Raimondi A, Caporali L, et al. Pathological mitophagy disrupts mitochondrial homeostasis in Leber's hereditary optic neuropathy. Cell Rep. 2022;40:111124 pubmed publisher
Oe Y, Kakuda K, Yoshimura S, Hara N, Hasegawa J, Terawaki S, et al. PACSIN1 is indispensable for amphisome-lysosome fusion during basal autophagy and subsets of selective autophagy. PLoS Genet. 2022;18:e1010264 pubmed publisher
Ke P, Chang C, Hsiao Y. Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN. Cells. 2022;11: pubmed publisher
Ravindran R, Velikkakath A, Narendradev N, Chandrasekharan A, Santhoshkumar T, Srinivasula S. Endosomal-associated RFFL facilitates mitochondrial clearance by enhancing PRKN/parkin recruitment to mitochondria. Autophagy. 2022;:1-14 pubmed publisher
Harbauer A, Hees J, Wanderoy S, Segura I, Gibbs W, Cheng Y, et al. Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy. Neuron. 2022;110:1516-1531.e9 pubmed publisher
Uesugi R, Ishii S, Matsuura A, Itakura E. Labeling and measuring stressed mitochondria using a PINK1-based ratiometric fluorescent sensor. J Biol Chem. 2021;297:101279 pubmed publisher
Kleele T, Rey T, Winter J, Zaganelli S, Mahecic D, Perreten Lambert H, et al. Distinct fission signatures predict mitochondrial degradation or biogenesis. Nature. 2021;593:435-439 pubmed publisher
Hung C, Lombardo P, Malik N, Brun S, Hellberg K, Van Nostrand J, et al. AMPK/ULK1-mediated phosphorylation of Parkin ACT domain mediates an early step in mitophagy. Sci Adv. 2021;7: pubmed publisher
Frison M, Faccenda D, Abeti R, Rigon M, Strobbe D, England Rendon B, et al. The translocator protein (TSPO) is prodromal to mitophagy loss in neurotoxicity. Mol Psychiatry. 2021;: pubmed publisher
Hollville E, Joers V, Nakamura A, Swahari V, TANSEY M, Moy S, et al. Characterization of a Cul9-Parkin double knockout mouse model for Parkinson's disease. Sci Rep. 2020;10:16886 pubmed publisher
Miyai T, Vasanth S, Melangath G, Deshpande N, Kumar V, Benischke A, et al. Activation of PINK1-Parkin-Mediated Mitophagy Degrades Mitochondrial Quality Control Proteins in Fuchs Endothelial Corneal Dystrophy. Am J Pathol. 2019;189:2061-2076 pubmed publisher
Sun X, Duan Y, Qin C, Li J, Duan G, Deng X, et al. Distinct multilevel misregulations of Parkin and PINK1 revealed in cell and animal models of TDP-43 proteinopathy. Cell Death Dis. 2018;9:953 pubmed publisher
Castagnaro S, Chrisam M, Cescon M, Braghetta P, Grumati P, Bonaldo P. Extracellular Collagen VI Has Prosurvival and Autophagy Instructive Properties in Mouse Fibroblasts. Front Physiol. 2018;9:1129 pubmed publisher
Palomo G, Granatiero V, Kawamata H, Konràd C, Kim M, Arreguin A, et al. Parkin is a disease modifier in the mutant SOD1 mouse model of ALS. EMBO Mol Med. 2018;10: pubmed publisher
Gu X, Qi Y, Feng Z, Ma L, Gao K, Zhang Y. Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway. Toxicol Lett. 2018;291:92-100 pubmed publisher
Ansari M, Khan N, Ahmad I, Haqqi T. Parkin clearance of dysfunctional mitochondria regulates ROS levels and increases survival of human chondrocytes. Osteoarthritis Cartilage. 2018;26:1087-1097 pubmed publisher
Zhang Y, Nguyen D, Olzomer E, Poon G, Cole N, Puvanendran A, et al. Rescue of Pink1 Deficiency by Stress-Dependent Activation of Autophagy. Cell Chem Biol. 2017;24:471-480.e4 pubmed publisher
Zhang Y, Su S, Zhao S, Yang Z, Zhong C, Chen X, et al. RIP1 autophosphorylation is promoted by mitochondrial ROS and is essential for RIP3 recruitment into necrosome. Nat Commun. 2017;8:14329 pubmed publisher
Hammerling B, Najor R, Cortez M, Shires S, Leon L, Gonzalez E, et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun. 2017;8:14050 pubmed publisher
Brennan L, Khoury J, Kantorow M. Parkin elimination of mitochondria is important for maintenance of lens epithelial cell ROS levels and survival upon oxidative stress exposure. Biochim Biophys Acta Mol Basis Dis. 2017;1863:21-32 pubmed publisher
Haack T, Ignatius E, Calvo Garrido J, Iuso A, Isohanni P, Maffezzini C, et al. Absence of the Autophagy Adaptor SQSTM1/p62 Causes Childhood-Onset Neurodegeneration with Ataxia, Dystonia, and Gaze Palsy. Am J Hum Genet. 2016;99:735-743 pubmed publisher
Di Sante G, Casimiro M, Pestell T, Pestell R. Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy. J Vis Exp. 2016;: pubmed publisher
Scott T, Wicker C, Suganya R, Dhar B, Pittman T, Horbinski C, et al. Polyubiquitination of apurinic/apyrimidinic endonuclease 1 by Parkin. Mol Carcinog. 2017;56:325-336 pubmed publisher
Swiader A, Nahapetyan H, Faccini J, D Angelo R, Mucher E, Elbaz M, et al. Mitophagy acts as a safeguard mechanism against human vascular smooth muscle cell apoptosis induced by atherogenic lipids. Oncotarget. 2016;7:28821-35 pubmed publisher
Hsieh C, Chu C, Lee H, Yuan Yang W. Triggering mitophagy with far-red fluorescent photosensitizers. Sci Rep. 2015;5:10376 pubmed publisher
Birsa N, Norkett R, Wauer T, Mevissen T, Wu H, Foltynie T, et al. Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase. J Biol Chem. 2014;289:14569-82 pubmed publisher
Drew B, Ribas V, Le J, Henstridge D, Phun J, Zhou Z, et al. HSP72 is a mitochondrial stress sensor critical for Parkin action, oxidative metabolism, and insulin sensitivity in skeletal muscle. Diabetes. 2014;63:1488-505 pubmed publisher
Hofmeister Brix A, Kollmann K, Langer S, Schultz J, Lenzen S, Baltrusch S. Identification of the ubiquitin-like domain of midnolin as a new glucokinase interaction partner. J Biol Chem. 2013;288:35824-39 pubmed publisher
Valentin Vega Y, Maclean K, Tait Mulder J, Milasta S, Steeves M, Dorsey F, et al. Mitochondrial dysfunction in ataxia-telangiectasia. Blood. 2012;119:1490-500 pubmed publisher
Yang J, Yang W. Spatiotemporally controlled initiation of Parkin-mediated mitophagy within single cells. Autophagy. 2011;7:1230-8 pubmed publisher
Narendra D, Tanaka A, Suen D, Youle R. Parkin is recruited selectively to impaired mitochondria and promotes their autophagy. J Cell Biol. 2008;183:795-803 pubmed publisher
product information
Catalog Number :
23955
Product Name :
YFP-Parkin
article :
doi10.1083/jcb.200809125
id3134
pubmed_id19029340
bacterial resistance :
Kanamycin
cloning :
backboneeYFP-C1 (clontech)
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
first ATG is deleted
growth strain :
Mammalian expression of human Park2 fused to EYFP
origin :
37
pi :
alt_names
Parkin
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAR-JP, LPRS2, PARK2, PDJ
genePRKN
id5071
genbank_ids
NM_004562
mutation
namePark2
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
locationN terminal on backbone
tageYFP
resistance markers :
604
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA