This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-TIE1
catalog :
23946
citations: 2
Reference
Griffin M, Sorum A, Miller G, Goddard W, Hsieh Wilson L. Sulfated glycans engage the Ang-Tie pathway to regulate vascular development. Nat Chem Biol. 2021;17:178-186 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23946
Product Name :
pDONR223-TIE1
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has a Stop codon. A set of 4 custom lentiviral vectors is available (pLX301 http://www.addgene.org/25895 , pLX302 http://www.addgene.org/25896 , pLX303 http://www.addgene.org/25897 , pLX304 http://www.addgene.org/25890 ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRP11-282K6.6, JTK14, TIE
geneTIE1
id7075
genbank_ids
mutation
nameTIE1
shRNA_sequence
size3417
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA