pDONR223-AXL | Addgene 23945 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223-AXL

catalog :

23945

citations: 7

Reference
Novoyatleva T, Rai N, Kojonazarov B, Veeroju S, Ben Batalla I, Caruso P, et al. Deficiency of Axl aggravates pulmonary arterial hypertension via BMPR2. Commun Biol. 2021;4:1002 pubmed publisher
Kuroda M, Halfmann P, Kawaoka Y. HER2-mediated enhancement of Ebola virus entry. PLoS Pathog. 2020;16:e1008900 pubmed publisher
Zhou Z, Li M, Zhang L, Zhao H, Sahin O, Chen J, et al. Oncogenic Kinase-Induced PKM2 Tyrosine 105 Phosphorylation Converts Nononcogenic PKM2 to a Tumor Promoter and Induces Cancer Stem-like Cells. Cancer Res. 2018;78:2248-2261 pubmed publisher
von Mässenhausen A, Brägelmann J, Billig H, Thewes B, Queisser A, Vogel W, et al. Implication of the Receptor Tyrosine Kinase AXL in Head and Neck Cancer Progression. Int J Mol Sci. 2016;18: pubmed publisher
Vouri M, Croucher D, Kennedy S, An Q, Pilkington G, Hafizi S. Axl-EGFR receptor tyrosine kinase hetero-interaction provides EGFR with access to pro-invasive signalling in cancer cells. Oncogenesis. 2016;5:e266 pubmed publisher
Brand T, Iida M, Stein A, Corrigan K, Braverman C, Luthar N, et al. AXL mediates resistance to cetuximab therapy. Cancer Res. 2014;74:5152-64 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher

product information

Catalog Number :

23945

Product Name :

pDONR223-AXL

article :

doi	10.1038/nature09627
id	3878
pubmed_id	21107320

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5005

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Donor vector

growth notes :

The ORF has a Stop codon. A set of 4 custom lentiviral vectors is available (pLX301 http://www.addgene.org/25895 , pLX302 http://www.addgene.org/25896 , pLX303 http://www.addgene.org/25897 , pLX304 http://www.addgene.org/25890 ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. Addgene QC NGS analysis identifies a Q31P and Q764R mutation relative to NP_068713.2. As the Kinase ORFeome collection has been generated by high throughput methods using RNA from different tissues and sources, it is possible that this is a tissue specific isoform.

origin :

pi :

alt_names

AXL receptor tyrosine kinase

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARK, AXL3, JTK11, Tyro7, UFO
gene	AXL
id	558

genbank_ids

mutation

Q31P, Q764R

name

AXL

shRNA_sequence

size

2658

species

9606

Homo sapiens