This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-YES1
catalog :
23938
citations: 4
Reference
Han T, Jiang S, Zheng H, Yin Q, Xie M, Little M, et al. Interplay between c-Src and the APC/C co-activator Cdh1 regulates mammary tumorigenesis. Nat Commun. 2019;10:3716 pubmed publisher
Wang Z, Chen X, Zhong M, Yang S, Zhou J, Klinkebiel D, et al. Cyclin-dependent kinase 1-mediated phosphorylation of YES links mitotic arrest and apoptosis during antitubulin chemotherapy. Cell Signal. 2018;52:137-146 pubmed publisher
Sun J, Wang X, Tang B, Liu H, Zhang M, Wang Y, et al. A tightly controlled Src-YAP signaling axis determines therapeutic response to dasatinib in renal cell carcinoma. Theranostics. 2018;8:3256-3267 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23938
Product Name :
pDONR223-YES1
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHsT441, P61-YES, Yes, c-yes
geneYES1
id7525
genbank_ids
mutation
nameYES1
shRNA_sequence
size1632
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA