This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-JAK1
catalog :
23932
citations: 7
Reference
Dykstra P, Rando T, Smolke C. Modulating myoblast differentiation with RNA-based controllers. PLoS ONE. 2022;17:e0275298 pubmed publisher
Xiang Q, Yang Z, Nicholas J. STAT and Janus kinase targeting by human herpesvirus 8 interferon regulatory factor in the suppression of type-I interferon signaling. PLoS Pathog. 2022;18:e1010676 pubmed publisher
Shomali W, Damnernsawad A, Theparee T, Sampson D, Morrow Q, Yang F, et al. A novel activating JAK1 mutation in chronic eosinophilic leukemia. Blood Adv. 2021;5:3581-3586 pubmed publisher
Xia Y, Chen S, Cui J, Wang Y, Liu X, Shen Y, et al. Berberine suppresses bladder cancer cell proliferation by inhibiting JAK1-STAT3 signaling via upregulation of miR-17-5p. Biochem Pharmacol. 2021;188:114575 pubmed publisher
Gruber C, Calis J, Buta S, Evrony G, Martin J, Uhl S, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity. 2020;53:672-684.e11 pubmed publisher
Kim H, Frederick D, Levesque M, Cooper Z, Feng Y, Krepler C, et al. Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell Rep. 2015;11:1458-73 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23932
Product Name :
pDONR223-JAK1
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
Janus kinase 1 (a protein tyrosine kinase)
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIIDE, JAK1A, JAK1B, JTK3
geneJAK1
id3716
genbank_ids
mutationR647H (numbering relative to NP_002218.1)
nameJAK1
shRNA_sequence
size3465
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA