This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-EPHA2
catalog :
23926
citations: 5
Reference
Kaibori Y, Katayama K, Tanaka Y, Ikeuchi M, Ogawa M, Ikeda Y, et al. Kinase activity-independent role of EphA2 in the regulation of M-phase progression. Exp Cell Res. 2020;395:112207 pubmed publisher
Valenzuela J, Perez F. Localized Intercellular Transfer of Ephrin-As by Trans-endocytosis Enables Long-Term Signaling. Dev Cell. 2019;: pubmed publisher
Zhou Z, Li M, Zhang L, Zhao H, Sahin O, Chen J, et al. Oncogenic Kinase-Induced PKM2 Tyrosine 105 Phosphorylation Converts Nononcogenic PKM2 to a Tumor Promoter and Induces Cancer Stem-like Cells. Cancer Res. 2018;78:2248-2261 pubmed publisher
Subbarayal P, Karunakaran K, Winkler A, Rother M, Gonzalez E, Meyer T, et al. EphrinA2 receptor (EphA2) is an invasion and intracellular signaling receptor for Chlamydia trachomatis. PLoS Pathog. 2015;11:e1004846 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23926
Product Name :
pDONR223-EPHA2
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
EPH receptor A2
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARCC2, CTPA, CTPP1, CTRCT6, ECK
geneEPHA2
id1969
genbank_ids
mutation
nameEPHA2
shRNA_sequence
size2931
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA