pDONR223-FGFR1 | Addgene 23922 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223-FGFR1

catalog :

23922

citations: 3

Reference
Albanese S, Parton D, Işık M, Rodríguez Laureano L, Hanson S, Behr J, et al. An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression. Biochemistry. 2018;57:4675-4689 pubmed publisher
Nakabayashi H, Kawahara M, Nagamune T. Cell-Surface Expression Levels Are Important for Fine-Tuning the Performance of Receptor Tyrosine Kinase-Based Signalobodies. Biotechnol J. 2017;12: pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher

product information

Catalog Number :

23922

Product Name :

pDONR223-FGFR1

article :

doi	10.1038/nature09627
id	3878
pubmed_id	21107320

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5005

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Donor vector

growth notes :

Please note that Addgene's sequencing results identifies mismatches at T631G and C1052T compared to the FGFR1 sequence provided, resulting in W211G and S351F mutations. The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

origin :

pi :

alt_names

fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1
gene	FGFR1
id	2260

genbank_ids

mutation

W211G and S351F compared to insert sequence provided

name

FGFR1

shRNA_sequence

size

2463

species

9606

Homo sapiens