This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-PDGFRA
catalog :
23892
citations: 9
Reference
Rogers M, Campa xf1 a M, Long R, Fantauzzo K. PDGFR dimer-specific activation, trafficking and downstream signaling dynamics. J Cell Sci. 2022;135: pubmed publisher
Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, et al. Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO Rep. 2022;23:e54041 pubmed publisher
Hamada T, Akahane T, Yokoyama S, Higa N, Kirishima M, Matsuo K, et al. An oncogenic splice variant of PDGFRα in adult glioblastoma as a therapeutic target for selective CDK4/6 inhibitors. Sci Rep. 2022;12:1275 pubmed publisher
Sun G, Chiuppesi F, Chen X, Wang C, Tian E, Nguyen J, et al. Modeling Human Cytomegalovirus-Induced Microcephaly in Human iPSC-Derived Brain Organoids. Cell Rep Med. 2020;1:100002 pubmed publisher
Drinane M, Yaqoob U, Yu H, Luo F, Greuter T, Arab J, et al. Synectin promotes fibrogenesis by regulating PDGFR isoforms through distinct mechanisms. JCI Insight. 2017;2: pubmed publisher
Gao Z, Daquinag A, Su F, Snyder B, Kolonin M. PDGFRα/PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes. Development. 2018;145: pubmed publisher
Chen L, Chai K. Proteolytic cleavages in the extracellular domain of receptor tyrosine kinases by membrane-associated serine proteases. Oncotarget. 2017;8:56490-56505 pubmed publisher
Liau B, Sievers C, Donohue L, Gillespie S, Flavahan W, Miller T, et al. Adaptive Chromatin Remodeling Drives Glioblastoma Stem Cell Plasticity and Drug Tolerance. Cell Stem Cell. 2017;20:233-246.e7 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23892
Product Name :
pDONR223-PDGFRA
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
platelet-derived growth factor receptor, alpha polypeptide
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD140A, PDGFR-2, PDGFR2
genePDGFRA
id5156
genbank_ids
mutationM260I
namePDGFRA
shRNA_sequence
size3270
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA