This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-NTRK1
catalog :
23891
citations: 4
Reference
Rukhlenko O, Halasz M, Rauch N, Zhernovkov V, Prince T, Wynne K, et al. Control of cell state transitions. Nature. 2022;609:975-985 pubmed publisher
Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, et al. Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO Rep. 2022;23:e54041 pubmed publisher
Chen L, Chai K. Proteolytic cleavages in the extracellular domain of receptor tyrosine kinases by membrane-associated serine proteases. Oncotarget. 2017;8:56490-56505 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23891
Product Name :
pDONR223-NTRK1
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
neurotrophic tyrosine kinase, receptor, type 1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA
geneNTRK1
id4914
genbank_ids
mutationV263L relative to NM_001012331
nameNTRK1
shRNA_sequence
size2373
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA