pDONR223-MET | Addgene 23889 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223-MET

catalog :

23889

citations: 6

Reference
Zhou Z, Li M, Zhang L, Zhao H, Sahin O, Chen J, et al. Oncogenic Kinase-Induced PKM2 Tyrosine 105 Phosphorylation Converts Nononcogenic PKM2 to a Tumor Promoter and Induces Cancer Stem-like Cells. Cancer Res. 2018;78:2248-2261 pubmed publisher
Kelso T, Porter D, Amaral M, Shokhirev M, Benner C, Hargreaves D. Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers. elife. 2017;6: pubmed publisher
Li Z, Ivanov A, Su R, Gonzalez Pecchi V, Qi Q, Liu S, et al. The OncoPPi network of cancer-focused protein-protein interactions to inform biological insights and therapeutic strategies. Nat Commun. 2017;8:14356 pubmed publisher
Manole S, Richards E, Meyer A. JNK Pathway Activation Modulates Acquired Resistance to EGFR/HER2-Targeted Therapies. Cancer Res. 2016;76:5219-28 pubmed publisher
Sullivan K, Padilla Just N, Henry R, Porter C, Kim J, Tentler J, et al. ATM and MET kinases are synthetic lethal with nongenotoxic activation of p53. Nat Chem Biol. 2012;8:646-54 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher

product information

Catalog Number :

23889

Product Name :

pDONR223-MET

article :

doi	10.1038/nature09627
id	3878
pubmed_id	21107320

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5005

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Donor vector

growth notes :

Please note that though the depositor's sequence suggests that this plasmid has MET transcript variant 1, a requesting scientist who sequenced the entire insert determined that this plasmid actually contains transcript variant 2 (NM_000245). The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

origin :

pi :

alt_names

met proto-oncogene (hepatocyte growth factor receptor)

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met
gene	MET
id	4233

genbank_ids

NM_000245

mutation

name

MET

shRNA_sequence

size

4173

species

9606

Homo sapiens